CAJ | 학술논문

本研究旨在通过测定低蛋白质饲粮中植物源血根碱替代色氨酸后仔猪门静脉血浆流率( PVPF)、氨基酸净吸收量及血清尿素氮净吸收量,探讨血根碱替代色氨酸对仔猪PVPF及门静脉氨基酸代谢的影响。试验选取12头20 kg左右的“杜×长×大”三元杂交公猪进行血液插管手术(颈动脉、门静脉和肠系膜静脉插管),随机分为4个处理,每个处理3头猪。4个处理分别为低蛋白质饲粮组(低蛋白质组)、低蛋白质不补充色氨酸饲粮组(缺色氨酸组)、低蛋白质不补充色氨酸添加血根碱饲粮组(血根碱组)和常规饲粮组。结果表明：1)猪PVPF会随着采食时间变化整体呈上升的趋势,在后期趋于稳定,缺色氨酸组6 h内平均PVPF比低蛋白质组、血根碱组和常规饲粮组均低,但差异不显著( P>0.05)。2)缺色氨酸组门静脉赖氨酸、色氨酸及精氨酸净吸收量显著低于蛋白质组、血根碱组和常规饲粮组( P<0.05),而低蛋白质组、血根碱组和常规饲粮组3组间门静脉赖氨酸、色氨酸及精氨酸净吸收量差异不显著( P>0.05)。缺色氨酸组门静脉必需氨基酸及总氨基酸净吸收量显著低于蛋白质组、血根碱组和常规饲粮组(P<0.05),而低蛋白质组和血根碱组间门静脉必需氨基酸及总氨基酸净吸收量差异不显著( P>0.05)。3)各组的血浆尿素氮的净吸收量相近,差异不显著( P>0.05)。由此可见,低蛋白质饲粮中不补充色氨酸,对PVPF和门静脉氨基酸净吸收量有一定影响；在不补充色氨酸添加血根碱后会相对提高PVPF,增加门静脉必需氨基酸和总氨基酸净吸收量,但不影响门静脉血浆尿素氮净吸收量。
본연구지재통과측정저단백질사량중식물원혈근감체대색안산후자저문정맥혈장류솔( PVPF)、안기산정흡수량급혈청뇨소담정흡수량,탐토혈근감체대색안산대자저PVPF급문정맥안기산대사적영향。시험선취12두20 kg좌우적“두×장×대”삼원잡교공저진행혈액삽관수술(경동맥、문정맥화장계막정맥삽관),수궤분위4개처리,매개처리3두저。4개처리분별위저단백질사량조(저단백질조)、저단백질불보충색안산사량조(결색안산조)、저단백질불보충색안산첨가혈근감사량조(혈근감조)화상규사량조。결과표명：1)저PVPF회수착채식시간변화정체정상승적추세,재후기추우은정,결색안산조6 h내평균PVPF비저단백질조、혈근감조화상규사량조균저,단차이불현저( P>0.05)。2)결색안산조문정맥뢰안산、색안산급정안산정흡수량현저저우단백질조、혈근감조화상규사량조( P<0.05),이저단백질조、혈근감조화상규사량조3조간문정맥뢰안산、색안산급정안산정흡수량차이불현저( P>0.05)。결색안산조문정맥필수안기산급총안기산정흡수량현저저우단백질조、혈근감조화상규사량조(P<0.05),이저단백질조화혈근감조간문정맥필수안기산급총안기산정흡수량차이불현저( P>0.05)。3)각조적혈장뇨소담적정흡수량상근,차이불현저( P>0.05)。유차가견,저단백질사량중불보충색안산,대PVPF화문정맥안기산정흡수량유일정영향；재불보충색안산첨가혈근감후회상대제고PVPF,증가문정맥필수안기산화총안기산정흡수량,단불영향문정맥혈장뇨소담정흡수량。
This study aimed to investigate the effects of botanical sanguinarine replacing tryptophan of low pro-tein diets on portal vein plasma flow rate and amino acids metabolism of piglets by testing portral vein plasma flow rate ( PVPF) , net absorptivity of amino acids and plasma urea nitrogen of piglets. Twelve growing pigs ( body wight about 20 kg, Duroc×Landrace ×Large White) were used to install blood intubation ( carotid arter-y, portal vein and mesenteric vein) , randomly divided into 4 treatment, each treatment with 3 pigs. Low pro-tein diet was the low-protein group, low protein diet without supplementary tryptophan was the lacking trypto-phan group, low protein diet with tryptophan replacement by sanguinarine was the sanguinarine group, normal diet was normal diet group. The results showed as follows: 1) PVPF was on the rise until stabilization in the later period with the feeding time, portal vein blood flow rate within 6 h of the lacking tryptophan group was lower than that of other groups, but no significant difference was observed ( P>0.05) . 2) The portal vein net absorption levels of lysine, tryptophan and arginine of the lacking tryptophan group was significantly lower than that of other groups ( P<0.05) , but no significant difference was observed between low-protein group, sangui-narine group and normal diets group (P>0.05). The portal vein net absorption levels of essential amino acid and total amino acid of the lacking tryptophan group was significantly lower than that of other groups ( P<0.05) , but no significant difference was observed between low-protein group and sanguinarine group ( P>0.05) . 3) The net absorption of the plasma urea nitrogen were closed in all groups, and no significant differ-ence was observed ( P>0.05) . In conclusion, the PVPF and net absorptivity of amino acids in portal vein of piglets are affected by feeding low protein diet without adding tryptophan;the PVPF, total amino acids in por-tal vein and net absorptivity of essential amino acids and total amino acids of piglets are increased by feeding low protein diet with sanguinarine but not trytophan, but the net absorptivity of plasma urea nitrogen is not af-fected.