CAJ | 학술논문

本试验旨在研究营养性复合添加剂对采食含霉变玉米饲粮断奶仔猪空肠黏膜结构与功能、氧化还原状态以及后肠微生物数量的影响。选用18头26日龄杜×长×大( DLY)断奶仔猪[(8.57±0.21) kg],随机分为3个组,每组6个重复,每个重复1头猪,分别饲喂对照组饲粮、霉变玉米组饲粮和霉变玉米+0.2%营养性复合添加剂饲粮,试验期30 d。结果发现：与对照组相比,霉变玉米极显著提高了断奶仔猪第30天血清二胺氧化酶( DAO )活性和空肠黏膜丙二醛(MDA)含量(P<0.01),显著或极显著降低了空肠黏膜总抗氧化能力(T-AOC)(P<0.01)、过氧化物歧化酶( SOD)( P<0.01)和谷胱甘肽过氧化物酶( GSH-Px)活性( P<0.05),以及盲肠和结肠中乳酸杆菌及盲肠总菌数量(P<0.01或P<0.05)。与霉变玉米组相比,霉变玉米+0.2%营养性复合添加剂显著或极显著降低了断奶仔猪第30天血清DAO活性( P<0.01)和空肠黏膜MDA含量(P<0.05),显著或极显著提高了空肠黏膜 GSH-Px活性(P<0.01)及咬合蛋白(occludin)( P<0.05)、闭合小环蛋白( ZO-1)( P<0.01)和溶质转运载体家族7成员1( SLC7A1) mRNA表达量( P<0.05)。上述结果表明,霉变玉米饲粮导致仔猪空肠通透性增加、抗氧化能力下降及后肠乳酸杆菌数量的降低,0.2%营养性复合添加剂可在一定程度缓解霉变玉米饲粮对仔猪空肠结构的损伤和脂质过氧化。
본시험지재연구영양성복합첨가제대채식함매변옥미사량단내자저공장점막결구여공능、양화환원상태이급후장미생물수량적영향。선용18두26일령두×장×대( DLY)단내자저[(8.57±0.21) kg],수궤분위3개조,매조6개중복,매개중복1두저,분별사위대조조사량、매변옥미조사량화매변옥미+0.2%영양성복합첨가제사량,시험기30 d。결과발현：여대조조상비,매변옥미겁현저제고료단내자저제30천혈청이알양화매( DAO )활성화공장점막병이철(MDA)함량(P<0.01),현저혹겁현저강저료공장점막총항양화능력(T-AOC)(P<0.01)、과양화물기화매( SOD)( P<0.01)화곡광감태과양화물매( GSH-Px)활성( P<0.05),이급맹장화결장중유산간균급맹장총균수량(P<0.01혹P<0.05)。여매변옥미조상비,매변옥미+0.2%영양성복합첨가제현저혹겁현저강저료단내자저제30천혈청DAO활성( P<0.01)화공장점막MDA함량(P<0.05),현저혹겁현저제고료공장점막 GSH-Px활성(P<0.01)급교합단백(occludin)( P<0.05)、폐합소배단백( ZO-1)( P<0.01)화용질전운재체가족7성원1( SLC7A1) mRNA표체량( P<0.05)。상술결과표명,매변옥미사량도치자저공장통투성증가、항양화능력하강급후장유산간균수량적강저,0.2%영양성복합첨가제가재일정정도완해매변옥미사량대자저공장결구적손상화지질과양화。
This experiment was conducted to study the protective effects of nutritional compound additive on je-junal mucosal structure and function, redox status and the numbers of bacteria in caecum and colon content of weaned piglets challenged-with feed contained corn naturally contaminated with mycotoxins. Eighteen“Duroc× Landrace×Large White” piglets were randomly allocated to control group, naturally contaminated corn group and naturally contaminated corn+0.2% nutritional compound additive group (6 replicates per group and 1 piglet per replicate) . The trial lasted for 30 d. The results showed that corn naturally contaminated with mycotoxins significantly increased the serum diamine oxidase (DAO) activity on day 30 (P<0.01) and the content of methane dicarboxylic aldehyde ( MDA) in jejuna mucosa ( P<0.01) , significantly decreased total antioxidation capability ( T-AOC) ( P<0.01) , superoxide dismutase ( SOD) ( P<0.01) and glutathione peroxidase ( GSH-Px) ( P<0.05) activities in jejuna mucosa, as well as the number of Lactobacillus in caecum and colon chyme and the number of total bacteria in caecum chyme compared to the control group ( P<0.05) . The 0.2% nutri-tional compound additive significantly decreased the serum DAO activity ( P<0.01) and the content of MDA in jejuna mucosa ( P<0.05) , significantly increaed GSH-Px activity in jejuna mucosa ( P<0.01) , and significant-ly up-regulated the mRNA expression of jejunal occludin ( P<0.05) , zonula-1 ( ZO-1) ( P<0.01) and solute carrier family 7 member 1 (SLC7A1) (P<0.05), in comparison with the corn naturally contaminated with mycotoxins group. Collectively, these results indicate that dietary mycotoxins can increase the intestinal perme-ability, and decrease the antioxidant capacity of jejunum mucosa and the numbers of Lactobacillus in caecum and colon contents. Meanwhile, dietary supplementation with 0.2% nutritional compound additive can alleviate the damage of jejuna mucosal structure and lipid peroxidation of jejunum in piglets fed mold-contaminated feeds, in a certain degree.