CAJ | 학술논문

目的研究PI3K/Akt信号通路对胰腺癌细胞PANC-1浸润迁移能力的影响，进一步探讨肿瘤相关巨噬细胞促进胰腺癌发生发展的分子机制。方法通过密度梯度离心法从健康成人外周血中分离单个核细胞，用IL-4体外诱导选择性激活的巨噬细胞(M2)。采用实时荧光定量PCR和Western blotting法检测胰腺癌PANC-1细胞PI3K、Akt mRNA 和蛋白表达水平的变化，利用Transwell侵袭实验与划痕实验观察细胞浸润迁移能力的变化。结果体外模拟胰腺癌微环境，将胰腺癌PANC-1细胞与不同激活状态的巨噬细胞共培养，证明M2可显著上调胰腺癌PANC-1细胞PI3K、Akt的mRNA和蛋白水平，共培养20 h后可明显促进胰腺癌PANC-1细胞的浸润与迁移能力。结论肿瘤相关巨噬细胞可通过PI3K/Akt信号通路促进胰腺癌细胞的浸润与迁移。
목적연구PI3K/Akt신호통로대이선암세포PANC-1침윤천이능력적영향，진일보탐토종류상관거서세포촉진이선암발생발전적분자궤제。방법통과밀도제도리심법종건강성인외주혈중분리단개핵세포，용IL-4체외유도선택성격활적거서세포(M2)。채용실시형광정량PCR화Western blotting법검측이선암PANC-1세포PI3K、Akt mRNA 화단백표체수평적변화，이용Transwell침습실험여화흔실험관찰세포침윤천이능력적변화。결과체외모의이선암미배경，장이선암PANC-1세포여불동격활상태적거서세포공배양，증명M2가현저상조이선암PANC-1세포PI3K、Akt적mRNA화단백수평，공배양20 h후가명현촉진이선암PANC-1세포적침윤여천이능력。결론종류상관거서세포가통과PI3K/Akt신호통로촉진이선암세포적침윤여천이。
Objective To explore the influence of PI3K/Akt signaling pathway on invasive and mi-gratory ability of pancreatic cancer cell line PANC-1, and further investigate the molecule mechanism of tu-mor-associated macrophages'promotion in the occurrence and development of pancreatic cancer. Methods Mononuclear cells were isolated from peripheral blood of healthy adults by density gradient centrifugation, and treated with IL-4 to obtain M2 in vitro. Expression of PI3K and Akt was evaluated by quantitative Real-time polymerase chain reaction and Western blotting. The ability of cellular invasion and migration was as-sessed by transwell chamber assay and wound healing assay. Results To simulate pancreatic cancer mi-croenvironment, we co-cultured pancreatic cancer PANC-1 cells and Ua or M2. The mRNA and protein lev-els of PI3K and Akt were remarkably increased analyzing by qRT-PCR and western blotting. In addition, transwell chamber assays and wound healing assays revealed that alternatively activated macrophages signif-icantly promoted the invasion and migration of PANC-1 cells in 20 h. Conclusion Tumor-associated macrophages may promote invasion and migration of pancreatic cancer cells via PI3K/Akt signaling pathway.