CAJ | 학술논문

本试验旨在研究饲料中硒含量对方格星虫( Sipunculus nudus Linnaeus)稚虫生长、体成分、组织硒含量及相关酶活性的影响。选取平均体重为(16.12±0.26) mg的方格星虫稚虫苗种7200条，随机分为6组，每组3个重复，每个重复400条。此6组方格星虫稚虫分别饲喂饲料中硒含量为0.19、0.37、0.63、0.82、1.06和1.29 mg/kg的饲料8周。结果表明：饲料中硒含量对方格星虫稚虫增重率、特定生长率和成活率都有显著的影响( P<0.05)。增重率和特定生长率随着饲料中硒含量的增加而上升，在1.29 mg/kg组达到最大，但成活率在此组则最低。饲料中硒含量对虫体的粗脂肪含量有显著影响(P<0.05)，虫体粗脂肪含量随着饲料中硒含量的增加而增加。虫体的水分、粗灰分和粗蛋白质含量各组间差异不显著( P>0.05)。饲料中硒含量对体壁和虫体硒含量都有显著影响( P<0.05)，两者都随着饲料中硒含量的增加而增加。此外，饲料中硒含量的变化还对虫体谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性有显著影响( P<0.05)，其中谷胱甘肽过氧化物酶的活性随着饲料中硒含量的增加而提高，而谷胱甘肽还原酶的活性随着饲料中硒含量的增加而降低。由此得出，饲料中适宜含量的硒可以促进方格星虫稚虫的生长，从而增加方格星虫的养殖产量。
본시험지재연구사료중서함량대방격성충( Sipunculus nudus Linnaeus)치충생장、체성분、조직서함량급상관매활성적영향。선취평균체중위(16.12±0.26) mg적방격성충치충묘충7200조，수궤분위6조，매조3개중복，매개중복400조。차6조방격성충치충분별사위사료중서함량위0.19、0.37、0.63、0.82、1.06화1.29 mg/kg적사료8주。결과표명：사료중서함량대방격성충치충증중솔、특정생장솔화성활솔도유현저적영향( P<0.05)。증중솔화특정생장솔수착사료중서함량적증가이상승，재1.29 mg/kg조체도최대，단성활솔재차조칙최저。사료중서함량대충체적조지방함량유현저영향(P<0.05)，충체조지방함량수착사료중서함량적증가이증가。충체적수분、조회분화조단백질함량각조간차이불현저( P>0.05)。사료중서함량대체벽화충체서함량도유현저영향( P<0.05)，량자도수착사료중서함량적증가이증가。차외，사료중서함량적변화환대충체곡광감태과양화물매화곡광감태환원매적활성유현저영향( P<0.05)，기중곡광감태과양화물매적활성수착사료중서함량적증가이제고，이곡광감태환원매적활성수착사료중서함량적증가이강저。유차득출，사료중괄의함량적서가이촉진방격성충치충적생장，종이증가방격성충적양식산량。
This trial was conducted to investigate the effects of dietary selenium content on growth, body com-position, tissue selenium content and related enzyme activities of juvenile peanut worm, Sipunculus nudus Lin-naeus. A total of 7 200 juvenile peanut worm with the average body weight of (16.12±0.26) mg were ran-domly divided into 6 groups with 3 replicates per group and 400 juveniles per replicate. The juveniles in those 6 groups were fed diets with six selenium contents (0.19, 0.37, 0.63, 0.82, 1.06 and 1.29 mg/kg, respec-tively) for 8 weeks. The results showed that the dietary selenium content had significant effects on weight gain rate ( WGR) , specific growth rate ( SGR) and survival ratio ( SR) of juvenile peanut worm ( P<0.05) . With the increase of dietary selenium content, the WGR and SGR were increased, and the highest values were ob-served in 1.29 mg/kg group, but the lowest value of SR was also found in this group. The dietary selenium content had a significant effect on body lipid content ( P<0.05) , the body lipid content showed an increasing trend as the dietary selenium content increasing. No significant differences were observed in body moisture, crude protein and ash contents among all groups (P>0.05). And dietary selenium content could significantly affected the contents of selenium in body wall and body ( P<0.05) , and those were increased with the dietary selenium content increasing. Moreover, dietary selenium content could significantly affected body glutathione peroxidas ( GSH-Px) and glutathione reductase ( GR) activities of juvenile peanut worm ( P<0.05) . With the of dietary selenium content increasing, the GSH-Px activity was increased and GR activity was declined. In conclusion, the suitable dietary selenium content can improve the growth of juvenile peanut worm, thus in-crease the cultured production of peanut worm.