CAJ | 학술논문

目的：通过检测2126例青壮年军人腰腿痛患者人类白细胞抗原B27（ HLA－B27）阳性率和表达强度，了解本地区军人强直性脊柱炎（ ankylosing spondylitis，AS）与HLA－B27抗原相关性，并初步探讨其对AS的临床诊断意义；通过检测确诊AS军人患者HLA－B27基因亚型，了解本地区军人HLA－B27基因亚型分布情况。方法用流式细胞仪分析2013年8月1日－2014年1月10日2126例因腰腿痛入南京军区南京总医院就诊的军人患者HLA－B27抗原表达情况；用荧光定量PCR法对其中确诊AS患者HLA－B27基因亚型进行检测分析。结果2126例患者中HLA－B27阳性者158例，阳性率为7．4％，男女患者HLA－B27抗原阳性率分别为7．1％和10．0％（P＜0．01）；33例HLA－B27阳性确诊患者中，B*2704和为B*2705为主要型别，分别占51．5％和42．4％。结论 HLA－B27对于AS仅有辅助诊断作用，不宜作为AS的常规筛选指标。
목적：통과검측2126례청장년군인요퇴통환자인류백세포항원B27（ HLA－B27）양성솔화표체강도，료해본지구군인강직성척주염（ ankylosing spondylitis，AS）여HLA－B27항원상관성，병초보탐토기대AS적림상진단의의；통과검측학진AS군인환자HLA－B27기인아형，료해본지구군인HLA－B27기인아형분포정황。방법용류식세포의분석2013년8월1일－2014년1월10일2126례인요퇴통입남경군구남경총의원취진적군인환자HLA－B27항원표체정황；용형광정량PCR법대기중학진AS환자HLA－B27기인아형진행검측분석。결과2126례환자중HLA－B27양성자158례，양성솔위7．4％，남녀환자HLA－B27항원양성솔분별위7．1％화10．0％（P＜0．01）；33례HLA－B27양성학진환자중，B*2704화위B*2705위주요형별，분별점51．5％화42．4％。결론 HLA－B27대우AS부유보조진단작용，불의작위AS적상규사선지표。
Objective To investigate the relationship and diagnostic significance between ankylosing spondylitis and HLA-B27 antigen in soldiers in one military district by detecting the positive rate and expression intensity of HLA-B27 antigen in 2126 young soldiers with low back pain, and evaluate its clinical significance.To investigate the distribution of genetic subtypes of AS by detecting genetic subtypes of HLA-B27 in confirmed soldiers.Methods The expression of HLA-B27 antigen of peripheral blood T lymphocytes in 2126 hospital admission patients was analyzed by flow cytometry.Genetic subtypes of HLA-B27 was determined by fluorescence quantitative polymerase chain reaction assay.Results 158 patients were positive in 2126 patients.The positive rate of antigen HLA-B27 was 7.4%, and the male and female patientsˊpositive rates of HLA-B27 antigen were 7.1% and 10.0%, respectively ( P<0.01).Among the 33 confirmed AS patients with HLA-B27 antigen positivity, B*2704 and B*2705 were the main type with account-ing for 51.5% and 42.4% respectively.Conclusion HLA-B27 is helpful for auxiliary diagnosis of AS, but is not suitable as a screening indictor of AS.