CAJ | 학술논문

目的：探讨卵泡刺激素β亚基（follicle stimulating hormone β－subunit，FSHβ）基因－211G→T（rs10835638）单核苷酸多态性与多囊卵巢综合征（ polysistic ovary syndrome，PCOS）易感性及临床内分泌代谢特征的关系。方法以2013年3－8月于解放军174医院妇科就诊的254例女性（100例PCOS和154例对照女性）为研究对象。收集所有研究对象年龄、身高、体重等资料，抽取静脉血检测六项生殖激素、空腹血糖、血脂等指标，采用PCR和DNA测序方法，检测FSHβ－211G→T的基因型分布，并分析其与PCOS临床内分泌代谢特征的关联。结果 FSHβ－211 G→T的基因型在两组中的分布差异有统计学意义（ P＜0．05）。 PCOS携带FSHβ－211G→T GT基因型的患者较GG基因型患者具有低FSH水平与高LH／FSH（P＜0．05）；对照组中携带FSHβ－211G→T GT基因型的具有高LH／FSH比值及高总胆固醇水平（P＜0．05）。结论 FSHβ－211G→T基因多态性可能是PCOS发病的原因之一。
목적：탐토란포자격소β아기（follicle stimulating hormone β－subunit，FSHβ）기인－211G→T（rs10835638）단핵감산다태성여다낭란소종합정（ polysistic ovary syndrome，PCOS）역감성급림상내분비대사특정적관계。방법이2013년3－8월우해방군174의원부과취진적254례녀성（100례PCOS화154례대조녀성）위연구대상。수집소유연구대상년령、신고、체중등자료，추취정맥혈검측륙항생식격소、공복혈당、혈지등지표，채용PCR화DNA측서방법，검측FSHβ－211G→T적기인형분포，병분석기여PCOS림상내분비대사특정적관련。결과 FSHβ－211 G→T적기인형재량조중적분포차이유통계학의의（ P＜0．05）。 PCOS휴대FSHβ－211G→T GT기인형적환자교GG기인형환자구유저FSH수평여고LH／FSH（P＜0．05）；대조조중휴대FSHβ－211G→T GT기인형적구유고LH／FSH비치급고총담고순수평（P＜0．05）。결론 FSHβ－211G→T기인다태성가능시PCOS발병적원인지일。
Objective To study the correlation between polymorphism of follicle stimulating hormone β-subunit ( FSHβ) gene-211G→T (rs10835638) and susceptibility and clinical endocrinal characteristics of polycystic ovary syndrome (PCOS).Meth-ods This research performed a case-control study.A total of 254 (100 patients with PCOS and 154 controls with tubal factor infertil-ity, matched for age) women were recruited at department of gynecology, 174 hospital of PLA, between March 2013 and August 2013 in the study.Age, body height, weight, reproductive hormone, Fasting blood glucose and fasting serum lipid were obtained.The geno-types of FSHβ-211 G→T polymorphism were determined for all cases by polymerase chain reaction ( PCR) and DNA sequencing.Re-sults The FSHβ-211 G→T GT genotype frequency in PCOS was significantly higher than that in controls (P<0.05).Within PCOS group, carriers of FSHβ-211 G→T GT genotype had lower FSH levels and higher LH/FSH (P<0.05).Within control group, carriers of FSHβ-211GT genotype had higher LH/FSH and total cholesterol (TC) levels (P<0.05).Conclusion Results suggested FSHβ-211G→T mutation might influence PCOS susceptibility and clinical endocrinal characteristics.FSHβ-211G→T is the possible cause of PCOS.