CAJ | 학술논문

目的：观察去卵巢对大鼠腰椎间盘退变的影响，建立大鼠腰椎间盘退变模型。方法：采用8个月龄健康雌性SD大鼠30只，随机分为空白组、假手术组、模型组，每组各10只。模型组行双侧卵巢切除术，假手术组在其双侧卵巢旁边分别取和卵巢同等大小的脂肪组织，空白组不做任何处理。造模后所有实验大鼠在同等条件下喂养，13周后全部处死，观察其腰椎间盘组织形态学、脊索细胞数量及髓核细胞超微结构的变化。结果：组织形态学评分及脊索细胞数量改变：模型组和空白组比较差异有统计学意义（P<0.05），空白组和假手术组比较差异无统计学意义（P>0.05）；透射电镜观察其髓核细胞的退变程度：模型组>空白组，空白组和假手术组退变程度相当。结论：采用去卵巢的方式能初步建立大鼠腰椎间盘退变模型。
목적：관찰거란소대대서요추간반퇴변적영향，건립대서요추간반퇴변모형。방법：채용8개월령건강자성SD대서30지，수궤분위공백조、가수술조、모형조，매조각10지。모형조행쌍측란소절제술，가수술조재기쌍측란소방변분별취화란소동등대소적지방조직，공백조불주임하처리。조모후소유실험대서재동등조건하위양，13주후전부처사，관찰기요추간반조직형태학、척색세포수량급수핵세포초미결구적변화。결과：조직형태학평분급척색세포수량개변：모형조화공백조비교차이유통계학의의（P<0.05），공백조화가수술조비교차이무통계학의의（P>0.05）；투사전경관찰기수핵세포적퇴변정도：모형조>공백조，공백조화가수술조퇴변정도상당。결론：채용거란소적방식능초보건립대서요추간반퇴변모형。
Objective:To observe effects of removing ovary on lumbar intervertebral disc, establishing rat model of lumbar intervetebral disc degeneration.Method:8-month-old healthy female SD rats were randomly divided into blank group, sham operation group and model group, 10 rats in each group.The blank group were subjected to no disposal. Rats of the sham operation group only underwent the resection of a bit fat.The model group underwent the resection of bilateral ovaries.all the rats were fed under the same conditions, All rats were sacrificed after feeding 13 weeks, the histomorphology of lumbar disc, notochord cells and the changes of the ultrastructure of nucleus pulposus cells were observed.Result:Tissue morphological grading and notochord cells quantity:there were significant differences between the model group and blank group (P<0.05), the difference in the blank group and the sham operation group was not significant(P>0.05).Level of degenerative nucleus pulposus cells observed under electron microscope:model group > blank group, It had no significant difference between the blank group and the sham operation group.Conclusion:Rat model of lumbar intervetebral disc degeneration was established preliminary through ovary resection of female rats.