CAJ | 학술논문

以鳕鱼肝脏为原料，采用木瓜蛋白酶水解提取鳕鱼肝油，选用提取率作为评价指标，通过单因素实验和响应面实验确定最佳的酶解提取工艺，并从鱼肝油的提取率、品质方面将其与传统淡碱水解法进行比较。实验结果表明，最佳酶解工艺条件为料液比1∶1．5、酶解 pH 6．5、加酶量3270 U ／g、酶解温度50℃、酶解时间2 h。在最佳酶解工艺条件下，鳕鱼肝油提取率可达到93．44％，且品质良好，酸价5．49 mg／g，碘价148．31 g／100 g，过氧化值7．49 meq ／kg。酶解法鳕鱼肝油提取率及品质均优于传统淡碱水解法。
이설어간장위원료，채용목과단백매수해제취설어간유，선용제취솔작위평개지표，통과단인소실험화향응면실험학정최가적매해제취공예，병종어간유적제취솔、품질방면장기여전통담감수해법진행비교。실험결과표명，최가매해공예조건위료액비1∶1．5、매해 pH 6．5、가매량3270 U ／g、매해온도50℃、매해시간2 h。재최가매해공예조건하，설어간유제취솔가체도93．44％，차품질량호，산개5．49 mg／g，전개148．31 g／100 g，과양화치7．49 meq ／kg。매해법설어간유제취솔급품질균우우전통담감수해법。
Co d oil was extracted by papain hydrolysis.The technology was optimized by single factors and response surface tests according to the extraction rate.The technology was compared with tradition-al dilute alkaline hydrolysis in the aspects of cod oil extraction rate and quality.The result showed that the optimum condition of enzymatic technology was as follows:ratio of solid to liquid 1 ∶1 .5,pH 6.5, the quantity of enzyme 3 270 U /g,temperature 50 ℃and enzymolysis time 2 h.The extraction rate of the optimal technology could reach 93.44% and the obtained fish oil had good quality with acid value 5.49 mg /g,iodine value 148.31 g /100 g and peroxide value 7.49 meq /kg.The extraction rate and quality of cod oil extracted by the enzymatic hydrolysis was prior to that extracted by the diluted alkaline hydrolysis method.