CAJ | 학술논문

根据溴酸钾（KBrO3）可在酸性条件下氧化3，3′，5，5′－四甲基联苯胺（TMB）而显色的原理，建立了小麦粉中溴酸钾定性检测的目视比色法和定量检测的分光光度法。研究了体系酸度、显色液用量、反应时间对显色的影响，并确立了最优化的实验条件。结果表明：在最优化实验条件下，显色体系的颜色随溴酸钾含量的升高而逐渐加深，溴酸钾浓度在0～4 mg／L 范围内符合比尔定律，线性回归方程为ΔA ＝0．2437C －0．0052，R2＝0．9993。可用于实际样品的测定，目视比色法可快速确定溴酸钾的含量范围，分光光度法的回收率为96．51％～101．70％，RSD 为1．79％～3．12％，能够满足市场监管的需求。
근거추산갑（KBrO3）가재산성조건하양화3，3′，5，5′－사갑기련분알（TMB）이현색적원리，건립료소맥분중추산갑정성검측적목시비색법화정량검측적분광광도법。연구료체계산도、현색액용량、반응시간대현색적영향，병학립료최우화적실험조건。결과표명：재최우화실험조건하，현색체계적안색수추산갑함량적승고이축점가심，추산갑농도재0～4 mg／L 범위내부합비이정률，선성회귀방정위ΔA ＝0．2437C －0．0052，R2＝0．9993。가용우실제양품적측정，목시비색법가쾌속학정추산갑적함량범위，분광광도법적회수솔위96．51％～101．70％，RSD 위1．79％～3．12％，능구만족시장감관적수구。
According to the theory that 3,3′,5,5′-tetramethylbenzidine(TMB)can be oxidized under acid condition by potassium bromate (KBrO3)and the principle of color reaction,potassium bromate content in wheat flour was detected by qualitative detection of visual colorimetry and quantitative detection of spectrophotometry.The effects of system acidity,color liquid dosage,reaction time on the change of color were studied,and the experimental conditions were optimized.Results showed that under the opti-mal experimental conditions,the color gradually deepened as the potassium bromate content rose,and the concentration of potassium bromate within the range of 0 ~4 mg/L in line with the beer′s law.The linear regression equation was ΔA =0.2437 C (mg/L)-0.0052 with R2 =0.9993.These methods can be ap-plied in practice,visual colorimetric method can determine the content of potassium bromate rapidly and the recovery rate of spectrophotometric method was 96.51% ~101.70%,RSD 1.79% ~3.12%,which can meet the needs of the supervise of food safety in the market.