CAJ | 학술논문

目的：探讨不同浓度孕激素对早孕人绒毛滋养层细胞表达基质金属蛋白酶10(ADAM10)、长型瘦素受体(Ob-R)及分泌可溶性的瘦素受体( SLR)、瘦素( LEP)的影响。方法培养原代人绒毛滋养层细胞,分别给予含有不同浓度的孕激素(0、100、150、200 ng/mL)培养24 h,检测细胞内ADAM10和Ob-R的相对表达量及上清液中SLR及LEP的含量。结果随着孕激素浓度的增加,早孕人绒毛滋养层细胞表达ADAM10的含量逐渐降低,组间两两比较有统计学差异(P<0.05)。随着孕激素浓度的增加,早孕人绒毛滋养层细胞表达Ob-R的含量逐渐增加,组间比较无统计学差异(P>0.05)。各组细胞上清液中SLR含量随着孕激素的浓度增高而逐渐降低,组间两两比较有统计学差异(P<0.05)。各组细胞上清液中LEP浓度随着孕激素浓度的增高而逐渐增高,组间两两比较有统计学差异(P<0.05)。采用Pearson检验发现SLR的表达与LEP的表达存在显著性负相关(R=-0.949, P<0.01)。结论孕激素可能通过影响ADAM10、SLR、LEP的表达而调控瘦素功能的发挥,参与妊娠期糖尿病(GDM)发生。
목적：탐토불동농도잉격소대조잉인융모자양층세포표체기질금속단백매10(ADAM10)、장형수소수체(Ob-R)급분비가용성적수소수체( SLR)、수소( LEP)적영향。방법배양원대인융모자양층세포,분별급여함유불동농도적잉격소(0、100、150、200 ng/mL)배양24 h,검측세포내ADAM10화Ob-R적상대표체량급상청액중SLR급LEP적함량。결과수착잉격소농도적증가,조잉인융모자양층세포표체ADAM10적함량축점강저,조간량량비교유통계학차이(P<0.05)。수착잉격소농도적증가,조잉인융모자양층세포표체Ob-R적함량축점증가,조간비교무통계학차이(P>0.05)。각조세포상청액중SLR함량수착잉격소적농도증고이축점강저,조간량량비교유통계학차이(P<0.05)。각조세포상청액중LEP농도수착잉격소농도적증고이축점증고,조간량량비교유통계학차이(P<0.05)。채용Pearson검험발현SLR적표체여LEP적표체존재현저성부상관(R=-0.949, P<0.01)。결론잉격소가능통과영향ADAM10、SLR、LEP적표체이조공수소공능적발휘,삼여임신기당뇨병(GDM)발생。
Objective To investigate the different concentrations of progesterone on the expression of a disintegrin and metalloprotease 10 (ADAM10),long form leptin receptor (Ob-R) and the secretion of soluble leptin receptor (SLR),leptin (LEP) in primary pregnancy human chorionic trophoblast cells. Methods Cultured primary human trophoblast cells and added in different concentrations of progesterone (0,100,150 and 200 ng/mL) for 24 hours. The relative expression of ADAM10 and Ob-R in the cells and the content of SLR and LEP in the supernatant were detected. Results With increasing concentrations of progesterone,early human trophoblast cells ADAM10 content gradual-ly decreased,the difference between the two groups was significant (P<0. 05). With increasing concentrations of progesterone,human chori-onic trophoblast cells in early pregnancy Ob-R expression levels increased, the difference was no statistically significant between each two groups (P>0. 05). SLR content of the cell supernatants as the concentration of progesterone increased and decreased,there are significant differences between each two groups (P>0. 05). LEP cell supernatant in each group with the increase of the concentration of progesterone concentration increased gradually between the two groups were significantly different(P>0. 05). Pearson’s test showed that the expression of expression SLR and LEP exists a significant negative correlation (R= -0. 949,P<0. 01). Conclusion Progesterone may influence the ex-pression of ADAM10,SLR and LEP by the regulation of leptin to participate GDM occurs.