局解手术学杂志
跼解手術學雜誌
국해수술학잡지
JOURNAL OF REGIONAL ANATOMY AND OPERATIVE SURGERY
2015年
3期
245-248
,共4页
罗金英%钟建鑫%舒绍兵%张洁%朱璨%张倩%汤玲%周继祥
囉金英%鐘建鑫%舒紹兵%張潔%硃璨%張倩%湯玲%週繼祥
라금영%종건흠%서소병%장길%주찬%장천%탕령%주계상
人牙周膜干细胞%Glil%增殖%成骨分化
人牙週膜榦細胞%Glil%增殖%成骨分化
인아주막간세포%Glil%증식%성골분화
human periodontal ligament stem cells%Glil%proliferation%osteogenic differentiation
目的：通过构建Glil基因腺病毒载体,以此上调人牙周膜干细胞( PDLSCs)中Glil基因的表达,从而探讨其对人PDLSCs增殖及成骨分化的影响。方法将Glil目的基因亚克隆至腺病毒载体,并将含目的基因的病毒转染PDLSCs细胞。 CCK-8法检测过表达Glil对PDLSCs增殖的影响,利用Western blot检测PDLSCs中Glil的表达水平及其成骨相关标志物ALP、Runx2的表达。结果成功构建了过表达Glil目的基因的腺病毒载体并转染PDLSCs,过表达Glil的PDLSCs与空载体组比较,增殖速率减慢(P=0.003),Western blot检测结果显示PDLSCs过表达Glil目的基因后可以高表达成骨分化标记蛋白ALP、Runx2,差异具有统计学意义(P<0.05)。结论过表达Glil基因会导致PDLSCs的增殖减慢,成骨相关标志物增加,说明Glil基因能使PDLSCs成骨分化能力增强。
目的：通過構建Glil基因腺病毒載體,以此上調人牙週膜榦細胞( PDLSCs)中Glil基因的錶達,從而探討其對人PDLSCs增殖及成骨分化的影響。方法將Glil目的基因亞剋隆至腺病毒載體,併將含目的基因的病毒轉染PDLSCs細胞。 CCK-8法檢測過錶達Glil對PDLSCs增殖的影響,利用Western blot檢測PDLSCs中Glil的錶達水平及其成骨相關標誌物ALP、Runx2的錶達。結果成功構建瞭過錶達Glil目的基因的腺病毒載體併轉染PDLSCs,過錶達Glil的PDLSCs與空載體組比較,增殖速率減慢(P=0.003),Western blot檢測結果顯示PDLSCs過錶達Glil目的基因後可以高錶達成骨分化標記蛋白ALP、Runx2,差異具有統計學意義(P<0.05)。結論過錶達Glil基因會導緻PDLSCs的增殖減慢,成骨相關標誌物增加,說明Glil基因能使PDLSCs成骨分化能力增彊。
목적：통과구건Glil기인선병독재체,이차상조인아주막간세포( PDLSCs)중Glil기인적표체,종이탐토기대인PDLSCs증식급성골분화적영향。방법장Glil목적기인아극륭지선병독재체,병장함목적기인적병독전염PDLSCs세포。 CCK-8법검측과표체Glil대PDLSCs증식적영향,이용Western blot검측PDLSCs중Glil적표체수평급기성골상관표지물ALP、Runx2적표체。결과성공구건료과표체Glil목적기인적선병독재체병전염PDLSCs,과표체Glil적PDLSCs여공재체조비교,증식속솔감만(P=0.003),Western blot검측결과현시PDLSCs과표체Glil목적기인후가이고표체성골분화표기단백ALP、Runx2,차이구유통계학의의(P<0.05)。결론과표체Glil기인회도치PDLSCs적증식감만,성골상관표지물증가,설명Glil기인능사PDLSCs성골분화능력증강。
Objective To up-regulate the expression of Glil gene in periodontal ligament stem cells ( PDLSCs) and to explore the effect of Glil gene on PDLSCs proliferation and osteogenesis differentiation by establishing Glil gene adenovirus vectors. Methods Subcloned Glil to viral backbone vector Adtrack-CMV and transfered the established vector to 293T cells, which was to acquire the virus particles. Trans-fected aim cells,namely PDLSCs,with these virus. Detected its effect on PDLSCs proliferation with CCK-8 assay, and detected the expression of Glil and the bone-related markers ALP and Runx2 through Western blot. Results An adenovirus vector, which were over expressed Glil gene, was successfully constructed and transfected to PDLSCs. Compared with the empty vector group and normal group, the over expressed one had a much slower proliferation rate in CCK-8 assay (P=0. 003). Western blot showed that ALP and Runx2 can be overexpressing os-teogenic differentiated after PDLSCs successfully transfected with the Glil gene. Conclusion Over expressing Glil gene would lead to a much slower proliferation rate in the PDLSCs and an increase of the bone-related markers. It is concluded that Glil can enhance the osteogenic dif-ferentiation capacity in PDLSCs.
