粮油食品科技
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SCIENCE AND TECHNOLOGY OF CEREALS,OILS AND FOODS
2015年
3期
53-58
,共6页
血柚皮%果胶%提取%十六烷基三甲基溴化铵%响应面优化
血柚皮%果膠%提取%十六烷基三甲基溴化銨%響應麵優化
혈유피%과효%제취%십륙완기삼갑기추화안%향응면우화
blood pomelo peel%pectin%extraction%CTAB%response surface optimization
为优化十六烷基三甲基溴化铵(CTAB)辅助提取血柚皮果胶工艺,在单因素实验基础上,选取 CTAB 浓度、pH、提取温度、提取时间为自变量,果胶提取率为响应值,采用 Box -Behnken 实验设计方法,研究各自变量及其交互作用对果胶提取率的影响,并利用 Design -Expert8.05b 软件,建立了果胶提取率与各因素的二次多项式模型。结果表明,CTAB 辅助提取血柚皮果胶的最佳工艺条件为 CTAB 浓度0.62%、pH 2.1、提取温度56℃、提取时间93 min,该条件下果胶提取率为20.85%,与预测值的相对误差为0.28%,表明该模型有效。所得的果胶黏度为58.2×10-3 Pa·S,甲氧基含量为6.47%,酯化度为38.9%,灰分为0.82%,总半乳糖醛酸果胶含量为85.7%,其主要理化性质完全符合 QB2484—2000标准,该果胶是一种低甲氧基果胶,可作为一种增稠剂使用。
為優化十六烷基三甲基溴化銨(CTAB)輔助提取血柚皮果膠工藝,在單因素實驗基礎上,選取 CTAB 濃度、pH、提取溫度、提取時間為自變量,果膠提取率為響應值,採用 Box -Behnken 實驗設計方法,研究各自變量及其交互作用對果膠提取率的影響,併利用 Design -Expert8.05b 軟件,建立瞭果膠提取率與各因素的二次多項式模型。結果錶明,CTAB 輔助提取血柚皮果膠的最佳工藝條件為 CTAB 濃度0.62%、pH 2.1、提取溫度56℃、提取時間93 min,該條件下果膠提取率為20.85%,與預測值的相對誤差為0.28%,錶明該模型有效。所得的果膠黏度為58.2×10-3 Pa·S,甲氧基含量為6.47%,酯化度為38.9%,灰分為0.82%,總半乳糖醛痠果膠含量為85.7%,其主要理化性質完全符閤 QB2484—2000標準,該果膠是一種低甲氧基果膠,可作為一種增稠劑使用。
위우화십륙완기삼갑기추화안(CTAB)보조제취혈유피과효공예,재단인소실험기출상,선취 CTAB 농도、pH、제취온도、제취시간위자변량,과효제취솔위향응치,채용 Box -Behnken 실험설계방법,연구각자변량급기교호작용대과효제취솔적영향,병이용 Design -Expert8.05b 연건,건립료과효제취솔여각인소적이차다항식모형。결과표명,CTAB 보조제취혈유피과효적최가공예조건위 CTAB 농도0.62%、pH 2.1、제취온도56℃、제취시간93 min,해조건하과효제취솔위20.85%,여예측치적상대오차위0.28%,표명해모형유효。소득적과효점도위58.2×10-3 Pa·S,갑양기함량위6.47%,지화도위38.9%,회분위0.82%,총반유당철산과효함량위85.7%,기주요이화성질완전부합 QB2484—2000표준,해과효시일충저갑양기과효,가작위일충증주제사용。
The process of CTAB -assisted extraction of pectin from blood pomelo peel was optimized. Based on the single -factor experiments,selecting concentration of CTAB,pH,temperature and time as independent variables and the extraction yield of pectin as response value,the effects of each independent variable and their interactions on the extraction yield were studied by Box -Behnken design.The quad-ratic multinomial model of each factor and the yield was set by Design -Expert 8.05b software.The re-sults showed that the optimal extraction conditions of pectin from blood pomelo peel were:CTAB concen-tration 0.62%,pH 2.1,temperature 56 ℃ and time 93 min.The yield of pectin could be up to 20.85% under the optimal extraction condition,the relative error was 0.28% compared to the predictive value,which indicated the feasible model fitted well with the experimental data.The pectin showed a vis-cosity of 58.2 ×10 -3 Pa·S,content of methoxy group 6.47%,degree of esterification 38.9%,ash content 0.82%,content of total galacturomic acid pectin 85.7%,which accorded with the standard of QB2484 -2000.The pectin was a kind of low methoxy pectin and could be used as thickener.