CAJ | 학술논문

建立了一种新的小麦脱氧雪腐镰刀菌烯醇（DON）高效液相色谱检测方法。采用聚乙二醇和水对小麦样品进行振荡提取，提取液分别用活性炭、柱层析硅胶、中性氧化铝、酸性氧化铝、碱性氧化铝净化，比较几种吸附剂对小麦 DON 净化效果。结果表明，用酸性氧化铝净化效果最好，除杂率为66．3％，净化液通过0．22μm 聚醚砜滤膜可直接用于 HPLC 检测；色谱条件：分离柱为 C18柱；流动相为甲醇∶水（20∶80）；波长为230 nm。三水平加标平均回收率为91．0％～103．7％，不同污染水平的小麦阳性样品精密度试验相对标准偏差（RSD）为2．3％～8．5％；与免疫亲和柱（IAC）净化方法比较，方法间误差为2．3％～8．4％。本方法具有操作简便、快速、准确、成本低，可满足小麦 DON 大宗样品定量测定的要求。
건립료일충신적소맥탈양설부렴도균희순（DON）고효액상색보검측방법。채용취을이순화수대소맥양품진행진탕제취，제취액분별용활성탄、주층석규효、중성양화려、산성양화려、감성양화려정화，비교궤충흡부제대소맥 DON 정화효과。결과표명，용산성양화려정화효과최호，제잡솔위66．3％，정화액통과0．22μm 취미풍려막가직접용우 HPLC 검측；색보조건：분리주위 C18주；류동상위갑순∶수（20∶80）；파장위230 nm。삼수평가표평균회수솔위91．0％～103．7％，불동오염수평적소맥양성양품정밀도시험상대표준편차（RSD）위2．3％～8．5％；여면역친화주（IAC）정화방법비교，방법간오차위2．3％～8．4％。본방법구유조작간편、쾌속、준학、성본저，가만족소맥 DON 대종양품정량측정적요구。
A new method was established to detect deoxynivalenol (DON)in wheat by high liquid chro-matography detection.The wheat samples were treated by oscillation with polyethylene glycol and water. Some kinds of adsorption materials like activated carbon,silica gel column chromatography,neutral alu-mina,acidic alumina,basic alumina were used to purify the extract,respectively,to compare the purif-ying effect on DON in wheat.The result showed that acidic alumina had the best purifying effect with the rate of removing impurities 66.3%.The purification liquid though 0.22 μm polyethersulfone membrane was detected by HPLC directly with C18 column,mobile phase of methanol∶water (20∶80)and wave-length of 230 nm.The percent recovery in three levels was 91.0%~103.7%.The range of the relative standard deviation (RSD)of positive wheat samples with different pollution levels was 2.3%~8.5%. Compared with the result of another method by immunoaffinity column cleanup,the deviation of two methods was 2.3%~8.4%.The characteristic of simple,rapid,accurate,and low cost makes this method suitable to meet the needs of quantitive detection of DON in wheat with large number of samples.