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SCIENCE AND TECHNOLOGY OF CEREALS,OILS AND FOODS
2015年
3期
90-94
,共5页
葛婵婵%熊犍%赵晨%汪洋%申琳%张晓琳
葛嬋嬋%熊犍%趙晨%汪洋%申琳%張曉琳
갈선선%웅건%조신%왕양%신림%장효림
玉米赤霉烯酮%芽孢杆菌%筛选%降解
玉米赤黴烯酮%芽孢桿菌%篩選%降解
옥미적매희동%아포간균%사선%강해
zearalenone%bacillus%screening%degradation
利用96孔板，通过菌株与毒素共培养的方法，从217株芽孢菌中，经初筛和复筛得到两株具有很好降解效果的菌株，即 T －246和 T －420，它们分别能够在6 h 和9 h 内将15μg／mL 的玉米赤霉烯酮完全降解。通过16S rDNA 序列分析鉴定，T －246和 T －420分别为同温层芽孢杆菌（Ba-cillus stratosphericus）和短小芽孢杆菌（Bacillus pumilus）。对其降解机理的初步研究表明，这两株菌对玉米赤霉烯酮都具有一定程度的吸附作用，但主要作用源于胞外酶的酶促降解。
利用96孔闆，通過菌株與毒素共培養的方法，從217株芽孢菌中，經初篩和複篩得到兩株具有很好降解效果的菌株，即 T －246和 T －420，它們分彆能夠在6 h 和9 h 內將15μg／mL 的玉米赤黴烯酮完全降解。通過16S rDNA 序列分析鑒定，T －246和 T －420分彆為同溫層芽孢桿菌（Ba-cillus stratosphericus）和短小芽孢桿菌（Bacillus pumilus）。對其降解機理的初步研究錶明，這兩株菌對玉米赤黴烯酮都具有一定程度的吸附作用，但主要作用源于胞外酶的酶促降解。
이용96공판，통과균주여독소공배양적방법，종217주아포균중，경초사화복사득도량주구유흔호강해효과적균주，즉 T －246화 T －420，타문분별능구재6 h 화9 h 내장15μg／mL 적옥미적매희동완전강해。통과16S rDNA 서렬분석감정，T －246화 T －420분별위동온층아포간균（Ba-cillus stratosphericus）화단소아포간균（Bacillus pumilus）。대기강해궤리적초보연구표명，저량주균대옥미적매희동도구유일정정도적흡부작용，단주요작용원우포외매적매촉강해。
Two bacillus T -246 and T -420,which possess very good degradation effect,have been screened from 217 strains of bacillus,which were cultured in 96 -well micro plate with strain and viral co -culture,by primary screen and rescreen.It was confirmed that 15 μg/mL zearalenones (ZEN) could be degraded by these strains within 6 h and 9 h,respectively.These two strains were identified as Bacillus stratosphericus and Bacillus pumilus based on 16S rDNA sequence analysis.In addition,the pre-liminary study on their degradation mechanism showed that they were able to bind ZEN,but the main effect of scavenging ZEN was due to the degradation activity of extracellular enzyme.
