现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
15期
1600-1602
,共3页
咽炎消合剂%慢性咽炎%动物模型%咽黏膜
嚥炎消閤劑%慢性嚥炎%動物模型%嚥黏膜
인염소합제%만성인염%동물모형%인점막
Yanyanxiao mixture%chronic pharyngitis%animal model%pharyngeal mucosa
目的：研究咽炎消合剂对慢性咽炎大鼠黏膜修复的作用及其机制。方法将48只 Wistar大鼠随机分为咽炎消合剂低剂量组、咽炎消合剂高剂量组、阳性药物组、抗生素组、模型组、空白对照组，每组8只。除空白对照组外，其余组采用5%氨水喷咽部制作慢性咽炎模型。造模成功后，咽炎消合剂低剂量和高剂量组分别给予咽炎消合剂1 mL/d 和2 mL/d灌胃，阳性药物组给予慢咽舒柠片溶解后1 mL/d 灌胃，抗生素组给予阿莫西林胶囊溶解后1 mL/d灌胃。20 d后取各组咽部黏膜及其下组织进行病理切片观察。结果治疗10 d后，咽炎消合剂高、低剂量组及阳性药物组动物的一般状态好转，以咽炎消合剂高、低剂量组更明显；咽炎消合剂低、高剂量组局部症状明显好转，摄食率降低等症状也随之消失，而阳性药物组的摄食率降低、骚动不安症状改善不明显；额镜观察其咽部组织基本接近正常，部分动物虽还有轻度的慢性充血，但其咽部黏膜颜色较润泽。给药20 d后，咽炎消合剂低剂量和高剂量组咽黏膜组织病理表现接近空白对照组，咽炎消合剂高剂量组黏膜上皮增生不明显，无炎细胞浸润，固有膜改变不大，肌层纤维近于排列规则，接近于空白对照组的黏膜形态。而抗生素组病理改变不大与模型组组织变化相近。结论咽炎消合剂通过抗炎、减轻炎性渗出、抑制组织增生等多环节发挥作用，疗效显著，证明了慢性咽炎脾胃学说理论的正确性。
目的：研究嚥炎消閤劑對慢性嚥炎大鼠黏膜脩複的作用及其機製。方法將48隻 Wistar大鼠隨機分為嚥炎消閤劑低劑量組、嚥炎消閤劑高劑量組、暘性藥物組、抗生素組、模型組、空白對照組，每組8隻。除空白對照組外，其餘組採用5%氨水噴嚥部製作慢性嚥炎模型。造模成功後，嚥炎消閤劑低劑量和高劑量組分彆給予嚥炎消閤劑1 mL/d 和2 mL/d灌胃，暘性藥物組給予慢嚥舒檸片溶解後1 mL/d 灌胃，抗生素組給予阿莫西林膠囊溶解後1 mL/d灌胃。20 d後取各組嚥部黏膜及其下組織進行病理切片觀察。結果治療10 d後，嚥炎消閤劑高、低劑量組及暘性藥物組動物的一般狀態好轉，以嚥炎消閤劑高、低劑量組更明顯；嚥炎消閤劑低、高劑量組跼部癥狀明顯好轉，攝食率降低等癥狀也隨之消失，而暘性藥物組的攝食率降低、騷動不安癥狀改善不明顯；額鏡觀察其嚥部組織基本接近正常，部分動物雖還有輕度的慢性充血，但其嚥部黏膜顏色較潤澤。給藥20 d後，嚥炎消閤劑低劑量和高劑量組嚥黏膜組織病理錶現接近空白對照組，嚥炎消閤劑高劑量組黏膜上皮增生不明顯，無炎細胞浸潤，固有膜改變不大，肌層纖維近于排列規則，接近于空白對照組的黏膜形態。而抗生素組病理改變不大與模型組組織變化相近。結論嚥炎消閤劑通過抗炎、減輕炎性滲齣、抑製組織增生等多環節髮揮作用，療效顯著，證明瞭慢性嚥炎脾胃學說理論的正確性。
목적：연구인염소합제대만성인염대서점막수복적작용급기궤제。방법장48지 Wistar대서수궤분위인염소합제저제량조、인염소합제고제량조、양성약물조、항생소조、모형조、공백대조조，매조8지。제공백대조조외，기여조채용5%안수분인부제작만성인염모형。조모성공후，인염소합제저제량화고제량조분별급여인염소합제1 mL/d 화2 mL/d관위，양성약물조급여만인서저편용해후1 mL/d 관위，항생소조급여아막서림효낭용해후1 mL/d관위。20 d후취각조인부점막급기하조직진행병리절편관찰。결과치료10 d후，인염소합제고、저제량조급양성약물조동물적일반상태호전，이인염소합제고、저제량조경명현；인염소합제저、고제량조국부증상명현호전，섭식솔강저등증상야수지소실，이양성약물조적섭식솔강저、소동불안증상개선불명현；액경관찰기인부조직기본접근정상，부분동물수환유경도적만성충혈，단기인부점막안색교윤택。급약20 d후，인염소합제저제량화고제량조인점막조직병리표현접근공백대조조，인염소합제고제량조점막상피증생불명현，무염세포침윤，고유막개변불대，기층섬유근우배렬규칙，접근우공백대조조적점막형태。이항생소조병리개변불대여모형조조직변화상근。결론인염소합제통과항염、감경염성삼출、억제조직증생등다배절발휘작용，료효현저，증명료만성인염비위학설이론적정학성。
Objective It is to observe the repairment effect of Yanyanxiao mixture( YYX)in pharyngeal mucosa of rats with chronic pharyngitis and its mechanism. Methods 48 Wistar rats were randomly divided into YYX low-dose group,YYX high-dose group,positive drug group( Manyanshuning),antibiotic group( amoxicillin),model group and black control group,8 cases in each. Except to the blank control group,all the other groups were received 5% ammonia for spraying in pharyngeal. After successful?animal model establishment,the rats were fed with YYX 1 mL/d and 2 mL/d orally in YYX low-dose group and YYX high-dose groups,and was given tablets dissolve Manyanshuning 1mL/d orally in the positive drug group,and was given amoxicillin liquid 1mL/d orally in antibiotic group. 20 days later,the pathology of pharyngeal mucosa tissue in each group was observed. Results 10 days after treatment,the general state of the animals in YYX high-dose group, low dose group and positive drug group were improved,and were better in YYX high-dose and low-dose groups. In YYX low-dose and high-dose group,the local symptoms were improved significantly,the symptoms of reduced feeding rate were disap-peared,and the feeding rate was reduced and the restlessness symptoms had no improved in positive drug group. Observation of frontal mirror showed that the pharyngeal tissue was closed to the normal animal,although parted of mild chronic conges-tion,but mucosal showed ruddy color. After 20 days of treatment,YYX high-dose group and low-dose group and pathological manifestations of mucosal tissue was closed to the black control group,epithelial hyperplasia was not obvious,and no inflam-matory cell infiltration,inherent film changed little,muscle fibers arranged more rules,these were closed to the blank control group. While the pathology change of antibiotics group was not big,and it was approximate to the model group. Conclusion YYX achieve a significant effect on chronic pharyngitis by anti-inflammatory,reducing inflammatory exudation,inhibiting hy-perplastic tissue and many other ways,it is proved the correctness of the theory of the spleen and stomach.
