CAJ | 학술논문

本文利用化学计量学交替三线性分解(ATLD)方法解析三维荧光数据，在有未知干扰组分共存的情况下，对中药升麻和人体血浆中升麻素的含量和回收率进行了测定，利用核一致诊断法判断得到的组分数是2。中药升麻和血浆体系的背景与升麻素有严重的重叠，用ATLD方法得到的解析光谱与真实光谱几乎完全重合，定量解析结果也令人满意。测定结果显示，标准曲线法和二阶标准加入法得到的中药升麻中升麻素的含量分别为0.061±0.003%和0.055±0.002%，回收率为89.8±4.7%，人体血浆中升麻素的回收率分别为102.7±1.6%。实验结果表明，此法可用于干扰组分共存下中药升麻和人体血浆中升麻素的快速准确定量分析。
본문이용화학계량학교체삼선성분해(ATLD)방법해석삼유형광수거，재유미지간우조분공존적정황하，대중약승마화인체혈장중승마소적함량화회수솔진행료측정，이용핵일치진단법판단득도적조분수시2。중약승마화혈장체계적배경여승마소유엄중적중첩，용ATLD방법득도적해석광보여진실광보궤호완전중합，정량해석결과야령인만의。측정결과현시，표준곡선법화이계표준가입법득도적중약승마중승마소적함량분별위0.061±0.003%화0.055±0.002%，회수솔위89.8±4.7%，인체혈장중승마소적회수솔분별위102.7±1.6%。실험결과표명，차법가용우간우조분공존하중약승마화인체혈장중승마소적쾌속준학정량분석。
A simple method for determination of Cimifugin in Cimicifugafoetida L. and human plasma even in the presence of other uncalibrated interferents by using excitation–emission matrix (EEM) fluorescence coupled with second-order calibration method based on alternating trilinear decomposition (ATLD) algorithm, the core consistency diagnostic (CORCONDIA) test indicated the correct number of components should be two. The EEM spectra of the analytes were overlapped with the background in Cimicifugafoetida L. and human plasma samples. The obtained results showed that the resolved spectra and real spectra of Cimifugin matched quite well with each other and the content of Cimifugin by standard curve and second-order standard addition method with component number N=2 are 0.061±0.003 % and 0.055±0.002 %, respectively. The average recovery is 89.8±4.7 %, and the average recovery in human plasma is 102.7±1.6 %. The experimental results show that the proposed method could be applied to simply quantify Cimifugin in Cimicifugafoetida L. and human plasma samples even in the presence of other uncalibrated interferents.