CAJ | 학술논문

为建立智利大麦染色体新的细胞遗传学标记，用多聚核苷酸探针对中国春－智利大麦双二倍体、1Hch和1HchS杂合附加系、4Hch、5Hch、6Hch和7Hch附加系进行原位杂交（FISH）分析。以Oligo－pTa535．1和Oligo－pSc119．2进行的双色原位杂交结果发现，前者能在1Hch ～7Hch染色体上产生不同信号，而后者能在除3Hch外的6对染色体末端产生杂交信号；以（GAA）8进行的单色原位杂交在智利大麦1Hch ～7Hch染色体上的杂交信号也各不相同。以上3个探针在智利大麦染色体上的杂交信号和小麦染色体信号不同，并且在染色体转移过程中未发现智利大麦多聚核苷酸序列明显删除现象。因此，可以用这3个探针进行原位杂交对小麦背景中的智利大麦染色体进行有效识别，并辅助利用智利大麦进行小麦染色体工程育种工作。
위건립지리대맥염색체신적세포유전학표기，용다취핵감산탐침대중국춘－지리대맥쌍이배체、1Hch화1HchS잡합부가계、4Hch、5Hch、6Hch화7Hch부가계진행원위잡교（FISH）분석。이Oligo－pTa535．1화Oligo－pSc119．2진행적쌍색원위잡교결과발현，전자능재1Hch ～7Hch염색체상산생불동신호，이후자능재제3Hch외적6대염색체말단산생잡교신호；이（GAA）8진행적단색원위잡교재지리대맥1Hch ～7Hch염색체상적잡교신호야각불상동。이상3개탐침재지리대맥염색체상적잡교신호화소맥염색체신호불동，병차재염색체전이과정중미발현지리대맥다취핵감산서렬명현산제현상。인차，가이용저3개탐침진행원위잡교대소맥배경중적지리대맥염색체진행유효식별，병보조이용지리대맥진행소맥염색체공정육충공작。
In order to develop new cytogenetic markers specific for Hordeum chilense chromosomes , the Chinese Spring-H.chilense amphipliod, heterozygous 1Hch﹠1HchS additon, 4Hch, 5Hch, 6Hch and 7Hch ad-ditions were analyzed by fluorescence in situ hybridization (FISH) using Oligo-pTa535.1, Oligo-pSc119.2 and ( GAA) 8 as probes .The results of double-color FISH using Oligo-pTa535 .1 and Oligo-pSc119 .2 as probes showed that the individual 1Hch ~7Hch chromosomes were painted by different signals except 3Hch chro-mosomes with Oligo-pSc119 .2 probe;the FISH using ( GAA) 8 as probe suggested that most of subtelomeric and centromere regions of 1Hch ~7Hch chromosomes were labeled by hybridization signals .The FISH signals of H.chilense chromosomes using these three probes were different from those of wheat chromosomes , and no oli-gonucleotide sequence elimination occurred during chromosome transformation .Therefore , FISH with these three probes could be used to identify H.chilense chromosomes in wheat background and be assistant to wheat breeding by chromosome engineering using the agronomical useful genes of H.chilense.