中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2015年
4期
273-276
,共4页
冠心病%血脂异常%阻遏蛋白质类%蛋白质丝氨酸苏氨酸激酶%细胞周期蛋白质类%多态现象,遗传
冠心病%血脂異常%阻遏蛋白質類%蛋白質絲氨痠囌氨痠激酶%細胞週期蛋白質類%多態現象,遺傳
관심병%혈지이상%조알단백질류%단백질사안산소안산격매%세포주기단백질류%다태현상,유전
Coronary disease%Dyslipidemias%Repressor proteins%Protein-serine-threonine kinases%Cell cycle proteins%Polymorphism,genetic
目的 探讨TRIB3基因rs2295490多态性与冠心病(CHD)血脂异常的关系.方法 病例对照研究.收集2013年10月至2014年10月于重庆医科大学附属永川医院心内科住院的220例冠心病患者作为观察组(平均年龄60.15岁,男性150例,女性70例),同时收集151名健康体检者做为对照组(平均年龄59.22岁,男性102名,女性49名),利用PCR和基因测序技术检测两组的TRIB3基因rs2295490多态性,同时对患者的生化指标如葡萄糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)进行检测,分析TRIB3基因rs2295490多态性在各组中的分布及各组不同基因型与生化指标的关系.各组间基因型和等位基因频率比较采用x2检验,生化指标间的比较采用独立样本t检验.结果 TRIB3基因rs2295490多态性在健康对照组中的分布是AA基因型频率为57％,AG为35％,GG为8％,A、G等位基因频率分别为75％、25％;CHD组AA、AG、GG基因型频率分别为50％、41％、9％,A、G等位基因频率分别是71％、29％.健康对照组和CHD组间基因型和等位基因分布频率差异没有统计学意义(x2=1.74,P=0.42;x2=1.26,P=0.243);CHD组AA基因型携带者的HDL-C水平明显高于AG+ GG基因型(t=-9.78,P=0.00),TG和LDL-C水平明显低于AG+ GG基因型(t=2.59,P=0.01;t =6.15,P=0.00).结论 TRIB3基因rs2295490多态性与CHD可能无相关性,但G等位基因可能与CHD患者的血脂代谢紊乱有关.
目的 探討TRIB3基因rs2295490多態性與冠心病(CHD)血脂異常的關繫.方法 病例對照研究.收集2013年10月至2014年10月于重慶醫科大學附屬永川醫院心內科住院的220例冠心病患者作為觀察組(平均年齡60.15歲,男性150例,女性70例),同時收集151名健康體檢者做為對照組(平均年齡59.22歲,男性102名,女性49名),利用PCR和基因測序技術檢測兩組的TRIB3基因rs2295490多態性,同時對患者的生化指標如葡萄糖(GLU)、總膽固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)進行檢測,分析TRIB3基因rs2295490多態性在各組中的分佈及各組不同基因型與生化指標的關繫.各組間基因型和等位基因頻率比較採用x2檢驗,生化指標間的比較採用獨立樣本t檢驗.結果 TRIB3基因rs2295490多態性在健康對照組中的分佈是AA基因型頻率為57％,AG為35％,GG為8％,A、G等位基因頻率分彆為75％、25％;CHD組AA、AG、GG基因型頻率分彆為50％、41％、9％,A、G等位基因頻率分彆是71％、29％.健康對照組和CHD組間基因型和等位基因分佈頻率差異沒有統計學意義(x2=1.74,P=0.42;x2=1.26,P=0.243);CHD組AA基因型攜帶者的HDL-C水平明顯高于AG+ GG基因型(t=-9.78,P=0.00),TG和LDL-C水平明顯低于AG+ GG基因型(t=2.59,P=0.01;t =6.15,P=0.00).結論 TRIB3基因rs2295490多態性與CHD可能無相關性,但G等位基因可能與CHD患者的血脂代謝紊亂有關.
목적 탐토TRIB3기인rs2295490다태성여관심병(CHD)혈지이상적관계.방법 병례대조연구.수집2013년10월지2014년10월우중경의과대학부속영천의원심내과주원적220례관심병환자작위관찰조(평균년령60.15세,남성150례,녀성70례),동시수집151명건강체검자주위대조조(평균년령59.22세,남성102명,녀성49명),이용PCR화기인측서기술검측량조적TRIB3기인rs2295490다태성,동시대환자적생화지표여포도당(GLU)、총담고순(TC)、감유삼지(TG)、고밀도지단백(HDL-C)、저밀도지단백(LDL-C)진행검측,분석TRIB3기인rs2295490다태성재각조중적분포급각조불동기인형여생화지표적관계.각조간기인형화등위기인빈솔비교채용x2검험,생화지표간적비교채용독립양본t검험.결과 TRIB3기인rs2295490다태성재건강대조조중적분포시AA기인형빈솔위57％,AG위35％,GG위8％,A、G등위기인빈솔분별위75％、25％;CHD조AA、AG、GG기인형빈솔분별위50％、41％、9％,A、G등위기인빈솔분별시71％、29％.건강대조조화CHD조간기인형화등위기인분포빈솔차이몰유통계학의의(x2=1.74,P=0.42;x2=1.26,P=0.243);CHD조AA기인형휴대자적HDL-C수평명현고우AG+ GG기인형(t=-9.78,P=0.00),TG화LDL-C수평명현저우AG+ GG기인형(t=2.59,P=0.01;t =6.15,P=0.00).결론 TRIB3기인rs2295490다태성여CHD가능무상관성,단G등위기인가능여CHD환자적혈지대사문란유관.
Objective To explore the correlation between the polymorphism of TRIB3 gene rs2295490 and coronary heart disease (CHD) with dyslipidemia.Methods In the case control study,we enrolled 220 CHD patients as observation group (60.15 years mean age,150 males and 70 females) and 151 age and gender matched healthy individuals as control group (59.22 years mean age,102 males and 49 fe males).Genotypes of TRIB3 gene rs2295490 polymorphisms in the two groups were detected by PCR and sequence-based testing.At the same time,blood glucose (GLU),total cholesterol (TC),triglyceride (TG),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) were measured.The correlations between the polymorphism of TRIB3 gene rs2295490 and CHD,biochemical indicators of two groups were analyzed,respectively.The comparison of genotype and allele frequency between two groups used x2 test,while the comparison of biochemical indexes between two groups used t test.Results In the control group,genotypic frequencies of AA,AG and GG of TRIB3 gene rs2295490 were 57％,35％ and 8％,and the frequencies of A and G alleles were 75％ and 25％,respectively.In the CHD group,genotypic frequencies of AA,AG and GG of TRIB3 gene rs2295490 were 50％,41％ and 9％,and the frequencies of A and G alleles were 71％ and 39％,respectively.There was no significant difference of genotype and allele frequencies of polymorphism of TRIB3 gene rs2295490 between CHD and healthy controls(x2 =1.74,P =0.42;x2 =1.26,P =0.243).In the CHD group,the HDL-C levels of the AA genotype were higher than those of AG + GG genotype (t =-9.78,P =0.00);The TG,LDL-C levels of the AA genotype were lower than those of AG + GG genotype (t =2.59,P =0.01;t =6.15,P =0.00).All the differences were statistically significant.Conclusions TRIB3 gene rs2295490 polymorphism has no correlation with CHD,but the G allele may be correlated with dyslipidemia in CHD patients.
