中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2015年
4期
251-255
,共5页
何大可%韩凤%宋小青%韩连书%高晓岚%金晶%吴洁%蔡嘉琳%顾学范
何大可%韓鳳%宋小青%韓連書%高曉嵐%金晶%吳潔%蔡嘉琳%顧學範
하대가%한봉%송소청%한련서%고효람%금정%오길%채가림%고학범
氨基酸类%肉碱%脑脊髓液%参考值%儿童
氨基痠類%肉堿%腦脊髓液%參攷值%兒童
안기산류%육감%뇌척수액%삼고치%인동
Amino acids%Carnitine%Cerebrospinal fluid%Reference values%Child
目的 初步建立儿童脑脊髓液氨基酸及酰基肉碱参考范围,为临床诊治遗传代谢病提供依据.方法 回顾性分析了采用串联质谱检测2010至2014年间来上海交通大学医学院附属新华医院儿科就诊的152例癫痫儿童脑脊髓液中氨基酸及酰基肉碱水平,分析其与年龄、性别的相关性;分析其中60例儿童脑脊髓液与血液氨基酸及酰基肉碱水平的差异.数据采用方差分析、Wilcoxon秩和检验及Spearman相关分析.结果 初步建立了儿童脑脊髓液氨基酸及酰基肉碱参考范围.血甲硫氨酸水平为18.66(15.24 ～ 24.11) μmol/L,戊二酰基肉碱水平为0.03(0.02 ～0.04) μmol/L,与脑脊髓液水平[23.41(13.80 ～28.58) μmol/L、0.03 (0.02～0.04) μmol/L]相近,差别无统计学意义(Z值分别为-0.69、-0.09,P均＞0.05);血精氨酸水平为3.82(2.00 ～8.36) mol/L,低于脑脊髓液水平[38.37(34.15 ～48.41) μmol/L],差别有统计学意义(Z=-6.74,P＜0.05);其余9种氨基酸、13种酰基肉碱血液浓度均高于脑脊髓液浓度,差异有统计学意义(P均＜0.05).结论 本研究初步建立了脑脊髓液氨基酸、酰基肉碱参考范围.
目的 初步建立兒童腦脊髓液氨基痠及酰基肉堿參攷範圍,為臨床診治遺傳代謝病提供依據.方法 迴顧性分析瞭採用串聯質譜檢測2010至2014年間來上海交通大學醫學院附屬新華醫院兒科就診的152例癲癇兒童腦脊髓液中氨基痠及酰基肉堿水平,分析其與年齡、性彆的相關性;分析其中60例兒童腦脊髓液與血液氨基痠及酰基肉堿水平的差異.數據採用方差分析、Wilcoxon秩和檢驗及Spearman相關分析.結果 初步建立瞭兒童腦脊髓液氨基痠及酰基肉堿參攷範圍.血甲硫氨痠水平為18.66(15.24 ～ 24.11) μmol/L,戊二酰基肉堿水平為0.03(0.02 ～0.04) μmol/L,與腦脊髓液水平[23.41(13.80 ～28.58) μmol/L、0.03 (0.02～0.04) μmol/L]相近,差彆無統計學意義(Z值分彆為-0.69、-0.09,P均＞0.05);血精氨痠水平為3.82(2.00 ～8.36) mol/L,低于腦脊髓液水平[38.37(34.15 ～48.41) μmol/L],差彆有統計學意義(Z=-6.74,P＜0.05);其餘9種氨基痠、13種酰基肉堿血液濃度均高于腦脊髓液濃度,差異有統計學意義(P均＜0.05).結論 本研究初步建立瞭腦脊髓液氨基痠、酰基肉堿參攷範圍.
목적 초보건립인동뇌척수액안기산급선기육감삼고범위,위림상진치유전대사병제공의거.방법 회고성분석료채용천련질보검측2010지2014년간래상해교통대학의학원부속신화의원인과취진적152례전간인동뇌척수액중안기산급선기육감수평,분석기여년령、성별적상관성;분석기중60례인동뇌척수액여혈액안기산급선기육감수평적차이.수거채용방차분석、Wilcoxon질화검험급Spearman상관분석.결과 초보건립료인동뇌척수액안기산급선기육감삼고범위.혈갑류안산수평위18.66(15.24 ～ 24.11) μmol/L,무이선기육감수평위0.03(0.02 ～0.04) μmol/L,여뇌척수액수평[23.41(13.80 ～28.58) μmol/L、0.03 (0.02～0.04) μmol/L]상근,차별무통계학의의(Z치분별위-0.69、-0.09,P균＞0.05);혈정안산수평위3.82(2.00 ～8.36) mol/L,저우뇌척수액수평[38.37(34.15 ～48.41) μmol/L],차별유통계학의의(Z=-6.74,P＜0.05);기여9충안기산、13충선기육감혈액농도균고우뇌척수액농도,차이유통계학의의(P균＜0.05).결론 본연구초보건립료뇌척수액안기산、선기육감삼고범위.
Objective To establish the reference values for some amino acids and acylcarnitines in cerebrospinal fluid of children,and provide evidence for the diagnosis of inherited metabolic diseases.Methods Retrospective study was made on 152 children with epilepsy collected from 2010 to 2014 in Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine.The levels of amino acids and acylcarnitines in the cerebrospinal fluid of these children were detected by tandem mass spectrometry,with their correlation with age and gender analysed,and their differences between blood and cerebrospinal fluid in 60 children were observed.Variance,Wilcoxon rank sum test and Spearman correlation were used for data analysis.Results These normal reference values of amino acids and acylcarnitines in cerebrospinal fluid of children were established.The Methionine and glutarylcarnitine levels in blood and cerebrospinal fluid had no difference [Methionine:18.66 (15.24-24.11) vs 23.41 (13.80-28.58) μmol/L,glutarylcamitine:0.03(0.02-0.04) vs 0.03(0.02-0.04) mol/L],(Z =-0.69,-0.09,all P ＞0.05)The arginine levels in blood was 3.82 (2.00-8.36) μmol/L,which was lower than cerebrospinal fluid [38.37(34.15-48.41) μmol/L],(Z =-6.74,P ＜0.05),while the other 9 kinds of amino acids and 13 kinds of acylcarnitines were higher in blood (all P ＜ 0.05).Conclusions The normal reference levels of amino acids and acylcarnitines in cerebrospinal fluid of children have been established,which may play a essential role in the diagnosis of inherited metabolic diseases.
