CAJ | 학술논문

目的：在大鼠最大电休克发作（ MES）及戊四唑惊厥（ MST）模型上，比较评价咪达唑仑与地西泮经鼻给药的抗惊厥作用。方法大鼠随机分为7组，分别为模型组、咪达唑仑鼻喷剂低、中、高剂量组和地西泮鼻喷剂低、中、高剂量组，每组10只。建立MES及MST模型，评价不同剂量咪达唑仑经鼻给药的抗惊厥作用，并与经典抗癫痫药物———地西泮比较，观察给药后72 h各组动物海马、皮质及杏仁核组织病理学变化。结果不同剂量咪达唑仑经鼻预防给药，均可剂量依赖性地对抗MES及MST；在等剂量给药条件下，其抗惊厥作用明显优于地西泮（P＜0．05或P＜0．01）。病理学结果显示，咪达唑仑与地西泮经鼻预防给药均可减轻癫痫所致海马、皮质及杏仁核神经元细胞的损伤，抑制小胶质细胞增多及炎性细胞浸润，细胞核固缩以及嗜神经现象减少。结论咪达唑仑经鼻给药，在经典癫痫模型上均具显著抗惊厥作用，并可显著抑制癫痫所致脑损伤。
목적：재대서최대전휴극발작（ MES）급무사서량궐（ MST）모형상，비교평개미체서륜여지서반경비급약적항량궐작용。방법대서수궤분위7조，분별위모형조、미체서륜비분제저、중、고제량조화지서반비분제저、중、고제량조，매조10지。건립MES급MST모형，평개불동제량미체서륜경비급약적항량궐작용，병여경전항전간약물———지서반비교，관찰급약후72 h각조동물해마、피질급행인핵조직병이학변화。결과불동제량미체서륜경비예방급약，균가제량의뢰성지대항MES급MST；재등제량급약조건하，기항량궐작용명현우우지서반（P＜0．05혹P＜0．01）。병이학결과현시，미체서륜여지서반경비예방급약균가감경전간소치해마、피질급행인핵신경원세포적손상，억제소효질세포증다급염성세포침윤，세포핵고축이급기신경현상감소。결론미체서륜경비급약，재경전전간모형상균구현저항량궐작용，병가현저억제전간소치뇌손상。
Objective To evaluate the anticonvulsant effect of midazolam and diazepam when administered nasally on maximal electroshock seizure and metrazol seizure threshold test models .Methods Rats were randomly divided into 7 groups:model group, low-dose, middle-dose and high-dose of midazolam nasal spray groups , diazepam nasal spray in low-dose, middle-dose and high-dose of midazolam nasal spray groups .After the establishment of the maximal electroshock seizure( MES) and metrazol seizure threshold test models ( MST) in rats, the anticonvulsant effects of different doses of midazolam and the clinically used antiepileptic drug diazepam were evaluated and compared .HE staining was used to observe the histopathological changes in the hippocampus , cortex and amygdala in rats .Results Significant anticonvulsant effects were observed on MES and MST in rats pretreated with different dosages of midazolam .In addition , the anticonvul-sant effects of midazolam were stronger than those of diazepam at the same dosage on MES and MST (P<0.05,P<0.01). Histopathological results showed that both midazolam and diazepam could effectively prevent the seizure -induced brain inju-ries, inhibit the increase of microglial cells and the inflammatory cell infiltration in the hippocampus, cortex and amygdala, and reduce the nucleus pycnosis and neuronophagia .Conclusion Midazolam has significantly anticonvulsant and neuropro-tective effects on different seizure models when administered nasally in rats .