CAJ | 학술논문

目的：研究伤寒沙门菌QseBC双组分调控系统对伤寒菌动力的调节作用。方法比较伤寒沙门菌野生株（WT）、qseB缺陷株（ΔqseB）和qseC缺陷株（ΔqseC）动力差异，选取主要鞭毛基因flhD进行实时定量RT－PCR检测，同时检测qseB的表达水平；构建qseB高表达株，比较其与空载体对照株动力和flhD表达差异。结果与WT相比，ΔqseB动力无变化，ΔqseC动力减弱；在ΔqseC中， flhD表达量下调至约1／4而qseB表达上调；qseB高表达株动力较WT减弱， flhD表达下调。结论 qseBC双组分调控系统能调节伤寒菌动力和主要鞭毛基因flhD的表达；qseB高表达时可抑制细菌动力。
목적：연구상한사문균QseBC쌍조분조공계통대상한균동력적조절작용。방법비교상한사문균야생주（WT）、qseB결함주（ΔqseB）화qseC결함주（ΔqseC）동력차이，선취주요편모기인flhD진행실시정량RT－PCR검측，동시검측qseB적표체수평；구건qseB고표체주，비교기여공재체대조주동력화flhD표체차이。결과여WT상비，ΔqseB동력무변화，ΔqseC동력감약；재ΔqseC중， flhD표체량하조지약1／4이qseB표체상조；qseB고표체주동력교WT감약， flhD표체하조。결론 qseBC쌍조분조공계통능조절상한균동력화주요편모기인flhD적표체；qseB고표체시가억제세균동력。
Objective To study the impact of QseBC on the motility of Salmonella enterica serovar Typhi ( S.Typhi ) . Methods The motility of wild-type ( WT) and null mutants (ΔqseB and ΔqseC) at mid-log phase was investigated by swimming assay.Quantitative RT-PCR was carried out to calculate the transcriptional variation of flhD and qseB among WT,ΔqseB andΔqseC.QseB overexpressing strain was constructed to compare its motility and flhD expression with the wild-type control.Results The result of motility assay showed that the motility of ΔqseB was similar to that of the WT strain , while the motility of ΔqseC was much lower than that of WT .qRT-PCR revealed that compared with WT , the expression of flhD was significantly decreased in ΔqseC while the expression of qseB was increased considerably .The motility of QseB overex-pressing strain was lower .Conclusion The expression of flhD may be regulated by QseBC which has an effect on the motil-ity of S.typhi, and the overexpression of QseB may inhibit the motility .