CAJ | 학술논문

目的：为揭示异常的可变剪接在肿瘤中发挥的作用，着重研究急性髓性白血病（ AML）与慢性髓性白血病（CML）内显著差异的可变剪接事件以及剪接异构体。方法基于CML（K562）和AML（THP1、HL－60）细胞系的全转录组测序数据，在全基因组范围内采用TopHat、MATS和Cufflinks计算识别可变剪接事件、重构剪接异构体和分析其差异表达。结果在AML和CML细胞间识别的130个基因在CML细胞系中特异性高表达，80个基因在AML两株细胞系内均表达上调。在CML与AML之间识别了337个差异表达的剪接异构体以及35个差异可变剪接事件。结论 AML与CML细胞系间存在显著差异的可变剪接事件和剪接异构体。白血病相关的可变剪接事件可作为其潜在的标志物或药物靶标。
목적：위게시이상적가변전접재종류중발휘적작용，착중연구급성수성백혈병（ AML）여만성수성백혈병（CML）내현저차이적가변전접사건이급전접이구체。방법기우CML（K562）화AML（THP1、HL－60）세포계적전전록조측서수거，재전기인조범위내채용TopHat、MATS화Cufflinks계산식별가변전접사건、중구전접이구체화분석기차이표체。결과재AML화CML세포간식별적130개기인재CML세포계중특이성고표체，80개기인재AML량주세포계내균표체상조。재CML여AML지간식별료337개차이표체적전접이구체이급35개차이가변전접사건。결론 AML여CML세포계간존재현저차이적가변전접사건화전접이구체。백혈병상관적가변전접사건가작위기잠재적표지물혹약물파표。
Objective To reveal the important role of aberrant splicing in cancer and to identify differential expressing isoforms and differential alternative splicing events between acute myeloid leukemia ( AML) and chronic myeloid leukemia (CML).Methods Based on whole transcriptome sequencing of CML (K562) and AML(THP1 and HL-60) cells,TopHat, MATS and Cufflinks were used to identify alternative splicing events and alternative splicing isoforms and analyze differenti -al expression of isoforms and genes .Results A total of 130 genes were identified to be highly expressed in CML and 80 genes in AML cells .Also, 337 differential expressed isoforms and 35 specific alternative splicing events between AML and CML cells were identified.Conclusion There are significant differences in alternative splicing isoforms and events between AML and CML cells.Leukemia-associated alternative splicing events can be used as potential tumor markers or drug targets.