中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
5期
674-677,682
,共5页
结直肠癌%细胞衍生因子%血管内皮生长因子
結直腸癌%細胞衍生因子%血管內皮生長因子
결직장암%세포연생인자%혈관내피생장인자
Colorectal cancer%Stromal cell-drived factor%Vascular endothelial growth factor
目的：研究VEGF、SDF-1及CXCR4在结直肠腺癌中的表达及其与临床病理学参数之间的相关性；探讨SDF-1／CXCR4轴与VEGF在结直肠腺癌发生发展浸润转移中的作用。方法：利用实时荧光定量PCR（ RT-qPCR）法及免疫组织化学SP法检测80例结直肠腺癌及其对应癌旁组织中SDF-1、CXCR4和VEGF mRNA及蛋白的表达。结果：在mRNA水平上，结直肠癌组中SDF-1、CXCR4、VEGF的表达显著高于对应的癌旁组织。免疫组化结果显示，肿瘤中c蛋白表达阳性率分别为78.7％、60％和66.3％高于对应癌旁组织的37.5％、42.5％和28.8％，差异显著（ P＜0.05）；结直肠癌中SDF-1表达水平与肿瘤细胞淋巴结转移、远处转移及TNM分期密切相关（P＜0.05），与患者的年龄、性别、肿瘤的大小、浸润深度等无关； CXCR4的表达与患者的淋巴结转移及TNM分期密切相关；SDF-1的表达与CXCR4及VEGF的表达成显著正相关。结论：SDF-1／CXCR4及VEGF在结直肠癌组织中高表达，并且与肿瘤生物学行为密切相关，提示其在肿瘤的发生发展浸润转移过程中发挥重要作用。
目的：研究VEGF、SDF-1及CXCR4在結直腸腺癌中的錶達及其與臨床病理學參數之間的相關性；探討SDF-1／CXCR4軸與VEGF在結直腸腺癌髮生髮展浸潤轉移中的作用。方法：利用實時熒光定量PCR（ RT-qPCR）法及免疫組織化學SP法檢測80例結直腸腺癌及其對應癌徬組織中SDF-1、CXCR4和VEGF mRNA及蛋白的錶達。結果：在mRNA水平上，結直腸癌組中SDF-1、CXCR4、VEGF的錶達顯著高于對應的癌徬組織。免疫組化結果顯示，腫瘤中c蛋白錶達暘性率分彆為78.7％、60％和66.3％高于對應癌徬組織的37.5％、42.5％和28.8％，差異顯著（ P＜0.05）；結直腸癌中SDF-1錶達水平與腫瘤細胞淋巴結轉移、遠處轉移及TNM分期密切相關（P＜0.05），與患者的年齡、性彆、腫瘤的大小、浸潤深度等無關； CXCR4的錶達與患者的淋巴結轉移及TNM分期密切相關；SDF-1的錶達與CXCR4及VEGF的錶達成顯著正相關。結論：SDF-1／CXCR4及VEGF在結直腸癌組織中高錶達，併且與腫瘤生物學行為密切相關，提示其在腫瘤的髮生髮展浸潤轉移過程中髮揮重要作用。
목적：연구VEGF、SDF-1급CXCR4재결직장선암중적표체급기여림상병이학삼수지간적상관성；탐토SDF-1／CXCR4축여VEGF재결직장선암발생발전침윤전이중적작용。방법：이용실시형광정량PCR（ RT-qPCR）법급면역조직화학SP법검측80례결직장선암급기대응암방조직중SDF-1、CXCR4화VEGF mRNA급단백적표체。결과：재mRNA수평상，결직장암조중SDF-1、CXCR4、VEGF적표체현저고우대응적암방조직。면역조화결과현시，종류중c단백표체양성솔분별위78.7％、60％화66.3％고우대응암방조직적37.5％、42.5％화28.8％，차이현저（ P＜0.05）；결직장암중SDF-1표체수평여종류세포림파결전이、원처전이급TNM분기밀절상관（P＜0.05），여환자적년령、성별、종류적대소、침윤심도등무관； CXCR4적표체여환자적림파결전이급TNM분기밀절상관；SDF-1적표체여CXCR4급VEGF적표체성현저정상관。결론：SDF-1／CXCR4급VEGF재결직장암조직중고표체，병차여종류생물학행위밀절상관，제시기재종류적발생발전침윤전이과정중발휘중요작용。
Objective:To study the differential expression of VEGF, SDF-1 and CXCR4 in colorectal cancer tissue and paracancerous tissues, and analyze the correlation between the three and the clinic pathological parameters of gastric cancer;to investigate the significance of the expression of VEGF,SDF-1 and CXCR4 in the progression of colorectal cancer.Methods:Immuno-histochemistry streptavidin-biotin complex( SABC) method and real-time Q-PCR were used to detect the operation resection of colorectal carcinoma and tumor-adjacent specimens of SDF-1,CXCR4 and VEGF expression.Results: On the mRNA level,compared with the adjacent tissues,the expression of SDF-1, CXCR4 and VEGF in colorectal cancer tissues was significant higher .The IHC′s results indicate that the positive rate of SDF-1,CXCR4 and VEGF in colorectal cancer were 78.7%,60% and 66.3%,the positive rate of adjacent tissues was 37.5%,42.5% and 28.8%, and there was statistically significant between expression and carcinoma adjacent tissue differences( P<0.05).There was statistically significant difference in the expression of SDF-1 with tumor metastasis,lymph node metastasis,TNM staging(P<0.05-P<0.01),while had no difference with gender,size and depth of invasion.There was statistically sig-nificant difference in the expression of CXCR4 with node metastasis,TNM in tumor staging ( P<0.05-P<0.01).It showed a significant negative correlation in SDF-1 expression and CXCR4,VEGF expression(P<0.01).Conclusion: It has a certain relationship between biological characteristics and SDF-1, CXCR4 and VEGF expression in colorectal cancer tissues.These results indicate that the expression of SDF-1,CXCR4 and VEGF play an important role in the tumorigenesis,progression,invasion and metastasis of colorectal cancer.
