CAJ | 학술논문

目的：大量研究表明，突触小体相关蛋白（synaptosomal-associated protein， SNAP）与多种肿瘤的发生发展密切相关。文中旨在筛选并验证在肺腺癌中特异性表达的突触小体相关蛋白47（synaptosomal-associated protein 47， SNAP47）并分析其与临床指标间的关系。方法应用表达谱基因芯片和肿瘤基因组图谱（ the cancer genome atlas ， TCGA）公共数据库筛选出特异性高表达于肺癌中的SNAP47，并选取江苏省肿瘤医院有完整临床资料的52例肺腺癌患者，对其癌组织及对应的癌旁肺组织应用实时定量逆转录聚合酶链反应检测SNAP47 mRNA的表达情况，并分析其与临床指标间的关系。结果52例肺腺癌组织中，41例（78．9％） SNAP47 mRNA表达水平明显高于对应的癌旁肺组织，差异有统计学意义（P＜0．05），且Ⅱ、Ⅲ期患者SNAP47 mRNA的表达水平（6．558±4．730）显著高于I期患者（2．718±2．370），N1＋N2组患者SNAP47 mRNA的表达水平（6．609±4．942）也明显高于N0组患者（3．360±2．987），差异均有统计学意义（P＜0．05）。结论 SNAP47 mRNA特异性高表达可能参与了肺腺癌的浸润及淋巴结转移，是潜在的肺癌诊疗靶点。
목적：대량연구표명，돌촉소체상관단백（synaptosomal-associated protein， SNAP）여다충종류적발생발전밀절상관。문중지재사선병험증재폐선암중특이성표체적돌촉소체상관단백47（synaptosomal-associated protein 47， SNAP47）병분석기여림상지표간적관계。방법응용표체보기인심편화종류기인조도보（ the cancer genome atlas ， TCGA）공공수거고사선출특이성고표체우폐암중적SNAP47，병선취강소성종류의원유완정림상자료적52례폐선암환자，대기암조직급대응적암방폐조직응용실시정량역전록취합매련반응검측SNAP47 mRNA적표체정황，병분석기여림상지표간적관계。결과52례폐선암조직중，41례（78．9％） SNAP47 mRNA표체수평명현고우대응적암방폐조직，차이유통계학의의（P＜0．05），차Ⅱ、Ⅲ기환자SNAP47 mRNA적표체수평（6．558±4．730）현저고우I기환자（2．718±2．370），N1＋N2조환자SNAP47 mRNA적표체수평（6．609±4．942）야명현고우N0조환자（3．360±2．987），차이균유통계학의의（P＜0．05）。결론 SNAP47 mRNA특이성고표체가능삼여료폐선암적침윤급림파결전이，시잠재적폐암진료파점。
[Abstract ] Objective Numerous studies had shown that synaptic-associated proteins (SNAPs) were closely related to the occurrence and development of tumors .The aim of this study was to investigate the expression of synaptosomal-associated protein 47 (SNAP47) and its correlation with the clinicopathological features in non-small cell lung cancer(NSCLC). Methods The expres-sions of SNAP family (SNAP23, SNAP25, SNAP29 and SNAP47) were extracted and analyzed through the gene expression microarray and the cancer genome atlas ( TCGA) data-base.SNAP47 mRNA expression in 52 cases of lung adenocarcinoma and their correspond-ing normal tissues were detected by quantitative real-time PCR ( qRT-PCR) . Results Among 52 cases of lung adenocarcinoma , SNAP47 mRNA expression levels of 41 cases(78.9%) were significantly higher than the adjacent lung tissue (P<0.05).The mRNA level of SNAP47 was associated with lymph node invasion and advanced clinical patho-logical stage .The mRNA levels of SNAP47 of patients in II/III stage were significantly higher than those of I stage patients ( 6.558 ± 4.730 vs 2.718 ±2.370, P<0.05).The mRNA levels of N1+N2 were higher than those of N0 (6.609 ±4.942 vs 3.360 ±2.987,P<0.05). Conclusion The high specificity of SNAP47 expression in lung cancer tissues might be associated with the invasion and lymph node metastasis of NSCLC , which is the potential therapeutic target of lung cancer .