CAJ | 학술논문

目的：糖尿病存在不同程度的氧化应激。文中通过复制1型糖尿病（type 1 diabetes mellitus， T1DM）大鼠模型，探讨氧化应激状态、血管内皮生长因子（ vascular endothelial growth factor ， VEGF）在糖尿病发生发展中的作用。方法40只雄性SD大鼠随机分为T1DM组（23只）和对照组组（17只）。一定剂量的链脲佐菌素复制T1DM大鼠模型，采用比色法检测血清中的总抗氧化能力（total anti oxidation capacity， T-AOC）、超氧化物歧化酶（super-oxide dismutase， SOD）、谷胱甘肽（Gluta-thione， GSH）和丙二醛（Malondialdehyde， MDA）；双抗体夹心ELISA法测定血清VEGF水平。结果①成模7周后，T1DM组大鼠空腹血糖明显高于对照组大鼠[（23．01±3．62） mmol／L vs （5．08±0．46）mmol／L， P＜0．05]；血清中GSH、SOD水平明显低于对照组大鼠[1．88（0．36）g／L vs （3．82±1．05）g／L，（192．06±34．57）U／mL vs （242．37±32．79）U／mL， P＜0．05]；血清MDA、VEGF水平明显升高[（4．94±0．63） nmol／mL vs （4．42±0．37） nmol／mL，（23．97±1．84） pg／mL vs （22．32±1．71） pg／mL， P＜0．05]；组间T-AOC水平差异无统计学意义（P＞0．05）。②实验大鼠空腹血糖与血清MDA、VEGF水平之间呈正相关（r＝0．51，0．47），与血清GSH、SOD水平呈负相关（r＝－0．71，－0．40）；血清VEGF与MDA的水平呈正相关（r＝0．32），与GSH水平呈负相关（r＝－0．34）。结论 T1DM发生发展过程中，体内过氧化水平增高、抗氧化机制受损，这种变化与糖尿病高血糖状态密切相关；高水平的VEGF与糖尿病高血糖状态及过氧化程度增高有关。
목적：당뇨병존재불동정도적양화응격。문중통과복제1형당뇨병（type 1 diabetes mellitus， T1DM）대서모형，탐토양화응격상태、혈관내피생장인자（ vascular endothelial growth factor ， VEGF）재당뇨병발생발전중적작용。방법40지웅성SD대서수궤분위T1DM조（23지）화대조조조（17지）。일정제량적련뇨좌균소복제T1DM대서모형，채용비색법검측혈청중적총항양화능력（total anti oxidation capacity， T-AOC）、초양화물기화매（super-oxide dismutase， SOD）、곡광감태（Gluta-thione， GSH）화병이철（Malondialdehyde， MDA）；쌍항체협심ELISA법측정혈청VEGF수평。결과①성모7주후，T1DM조대서공복혈당명현고우대조조대서[（23．01±3．62） mmol／L vs （5．08±0．46）mmol／L， P＜0．05]；혈청중GSH、SOD수평명현저우대조조대서[1．88（0．36）g／L vs （3．82±1．05）g／L，（192．06±34．57）U／mL vs （242．37±32．79）U／mL， P＜0．05]；혈청MDA、VEGF수평명현승고[（4．94±0．63） nmol／mL vs （4．42±0．37） nmol／mL，（23．97±1．84） pg／mL vs （22．32±1．71） pg／mL， P＜0．05]；조간T-AOC수평차이무통계학의의（P＞0．05）。②실험대서공복혈당여혈청MDA、VEGF수평지간정정상관（r＝0．51，0．47），여혈청GSH、SOD수평정부상관（r＝－0．71，－0．40）；혈청VEGF여MDA적수평정정상관（r＝0．32），여GSH수평정부상관（r＝－0．34）。결론 T1DM발생발전과정중，체내과양화수평증고、항양화궤제수손，저충변화여당뇨병고혈당상태밀절상관；고수평적VEGF여당뇨병고혈당상태급과양화정도증고유관。
O bjective Different degrees of oxidative stress may exist in patients with diabetes mellitus (DM).The aim of this study was to explore the roles of oxidative stress and the level of vascular endothelial growth factor ( VEGF) in the development and progression of DM in the rat model of type 1 DM (T1DM). Methods We established the T1DM model in 23 male SD rats and in-cluded another 17 in the control group .We determined the total anti-oxidation capacity ( T-AOC) and the levels of super-oxide dis-mutase (SOD), glutathione (GSH), and malondialdehyde (MDA) by chromatometry, and measured that of VEGF by ELISA. Results At 7 weeks after modeling, the level of fasting blood glucose(FBG) was significantly increased in the T1DM rats as com-pared with the controls ([23.01 ±3.62] vs [5.08 ±0.46] mmol/L, P<0.05); the levels of GSH and SOD were markedly lower (18.8 [0.36] vs [3.82 ±1.05] g/L and [192.06 ±34.57] vs [242.37 ±32.79] U/mL, both P<0.05) while those of serum MDA and VEGF remarkably higher in the T1DM than in the control rats ([4.94 ±0.63] vs [4.42 ±0.37] nmolm/L and [23.97 ±1.84] vs [22.32 ±1.71] pg/mL, both P<0.05);no statistically significant differences were found in T -AOC between the two groups (P>0.05).FBG was correlated positively with MDA (r=0.51) and VEGF (r=0.47) but negatively with GSH (r=-0.71) and SOD (r=-0.40), while serum VEGF positively with MDA (r=0.32) and negatively with GSH ( r=-0.34 ) in the experimental rats . Conclusion Peroxidation is increased and the anti-oxidation mecha-nism weakened during the development and progression of T 1DM, which is closely related to hyperglycemia .High VEGF is associated with hyperglycemia and enhanced peroxidation .