中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
5期
638-642
,共5页
多西他赛%希罗达%乳腺癌癌前病变%微血管密度%转化生长因子%血管内皮生长因子
多西他賽%希囉達%乳腺癌癌前病變%微血管密度%轉化生長因子%血管內皮生長因子
다서타새%희라체%유선암암전병변%미혈관밀도%전화생장인자%혈관내피생장인자
Docetaxel%Capecitabine%Mammary precancerous lesion%MVD%TGF-α%VEGF
目的：探讨多西他赛联合希罗达对乳腺癌癌前病变组织微血管密度、转化生长因子-1及血管内皮生长因子表达的影响。方法：将350只SD大鼠随机分为7组。采用二甲基苯蒽诱导大鼠乳腺癌癌前病变，进行4周干预治疗，从第8周开始开始将大鼠处死并进行病理学分析。采用免疫组化检测MVD和TGF-α的表达情况、原位杂交法测定VEGF mRNA的表达。结果：造模组的死亡率和癌前病变发生率均明显较高；多西他赛联合希罗达的大剂量和中剂量组的癌前病变的发生率明显较低；各组不同病变类型组织中的MVD、TGF-α及VEGF mRNA的阳性表达率均呈递增趋势；在非典型增生组中，大剂量组、中剂量组及多西他赛组的MVD与TGF-α的阳性表达率均明显较低，各干预组的VEGF mRNA的阳性率均明显较低；对于VEGF mRNA的阳性率的非典型增生，大剂量组中的VEGF mRNA的阳性率明显低于小剂量组（ P＜0.05）。结论：多西他赛联合希罗达能够抑制乳腺癌的癌前病变的血管生成及相关调控因子TGF-α的表达，降低DMBA诱导的大鼠的乳腺癌癌前病变组织中的VEGF mRNA表达量。
目的：探討多西他賽聯閤希囉達對乳腺癌癌前病變組織微血管密度、轉化生長因子-1及血管內皮生長因子錶達的影響。方法：將350隻SD大鼠隨機分為7組。採用二甲基苯蒽誘導大鼠乳腺癌癌前病變，進行4週榦預治療，從第8週開始開始將大鼠處死併進行病理學分析。採用免疫組化檢測MVD和TGF-α的錶達情況、原位雜交法測定VEGF mRNA的錶達。結果：造模組的死亡率和癌前病變髮生率均明顯較高；多西他賽聯閤希囉達的大劑量和中劑量組的癌前病變的髮生率明顯較低；各組不同病變類型組織中的MVD、TGF-α及VEGF mRNA的暘性錶達率均呈遞增趨勢；在非典型增生組中，大劑量組、中劑量組及多西他賽組的MVD與TGF-α的暘性錶達率均明顯較低，各榦預組的VEGF mRNA的暘性率均明顯較低；對于VEGF mRNA的暘性率的非典型增生，大劑量組中的VEGF mRNA的暘性率明顯低于小劑量組（ P＜0.05）。結論：多西他賽聯閤希囉達能夠抑製乳腺癌的癌前病變的血管生成及相關調控因子TGF-α的錶達，降低DMBA誘導的大鼠的乳腺癌癌前病變組織中的VEGF mRNA錶達量。
목적：탐토다서타새연합희라체대유선암암전병변조직미혈관밀도、전화생장인자-1급혈관내피생장인자표체적영향。방법：장350지SD대서수궤분위7조。채용이갑기분은유도대서유선암암전병변，진행4주간예치료，종제8주개시개시장대서처사병진행병이학분석。채용면역조화검측MVD화TGF-α적표체정황、원위잡교법측정VEGF mRNA적표체。결과：조모조적사망솔화암전병변발생솔균명현교고；다서타새연합희라체적대제량화중제량조적암전병변적발생솔명현교저；각조불동병변류형조직중적MVD、TGF-α급VEGF mRNA적양성표체솔균정체증추세；재비전형증생조중，대제량조、중제량조급다서타새조적MVD여TGF-α적양성표체솔균명현교저，각간예조적VEGF mRNA적양성솔균명현교저；대우VEGF mRNA적양성솔적비전형증생，대제량조중적VEGF mRNA적양성솔명현저우소제량조（ P＜0.05）。결론：다서타새연합희라체능구억제유선암적암전병변적혈관생성급상관조공인자TGF-α적표체，강저DMBA유도적대서적유선암암전병변조직중적VEGF mRNA표체량。
Objective:To explore the reversal effect of Docetaxel and Capecitabine on rat breast precancerous from angiogenesis and expression of related regulatory factors VEGF mRNA in rats.Methods: 350 SD rats were divided into 7 groups.Model of rats mammary was induced by DMBA and was treated by Docetaxel and Capecitabine for 4 weeks.All of them were killed from 8th week.The microvascular were detected in the specimens, examination of histopathology was performed and the expression of VEGF mRNA was measured by in situ hybridization.Results:The rat mortality and the incidence of precancerous lesions increased obviously, and the incidence of precancerous lesion of the high-dose group and the middle dose reduced.The positive rate of the expression of MVD,TGF-αand VEGF mRNA tend to increase in all the groups.The positive-cell rate of VEGF mRNA of Docetaxel,Capecitabine, Docetaxel and Capecitabine in ADH was lower than in the model group,and the positive-cell rate of VEGF mRNA of Docetaxel and Capecitabine was lower than Capecitabine and Docetaxel separately.Conclusion: Combination of Docetaxel and Capecitabinecan decrease the expression of VEGF mRNA in precancerouslesion of rats mammary.
