中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
5期
607-612
,共6页
印双红%张俊波%陈小林%徐芳洁%侯隽%吴向未%陈雪玲%姚元勇%邢明明%杨文才
印雙紅%張俊波%陳小林%徐芳潔%侯雋%吳嚮未%陳雪玲%姚元勇%邢明明%楊文纔
인쌍홍%장준파%진소림%서방길%후준%오향미%진설령%요원용%형명명%양문재
细粒棘球蚴%T细胞亚群%Treg细胞%NKG2D
細粒棘毬蚴%T細胞亞群%Treg細胞%NKG2D
세립극구유%T세포아군%Treg세포%NKG2D
Echinococcus granulosus%T-lymphocyte subsets%Treg cells%NKG2D
目的：观察TGF-β1对细粒棘球蚴体外与BALB／c小鼠T淋巴细胞培养的影响。方法：阻断剂组：BALB／c小鼠脾细胞＋细粒棘球蚴＋SB525334共培养；对照组：BALB／c小鼠脾细胞＋细粒棘球蚴＋PBS共培养；空白对照：BALB／c小鼠脾细胞＋RPMI-1640培养基＋SB525334共培养。方法：48 h收集淋巴细胞，流式细胞仪检测T淋巴细胞亚群、CD4＋CD25＋T细胞的数量、NK细胞的数量及其活性受体NKG2D的表达；乳酸脱氢酶法检测NK细胞的杀伤活性。结果：体外阻断TGF-β1受体后，引起细粒棘球蚴介导的CD4＋T细胞数量升高、CD8＋T细胞数量降低、CD4＋／CD8＋T细胞比值升高、CD4＋CD25＋T细胞数量减少、NK细胞活性受体NKG2D的表达增加、NK细胞对靶细胞Yac-1的裂解率增加和NK细胞活性及其活性受体NKG2D成正相关。结论：体外阻断TGF-β1受体可提高T淋巴细胞免疫，从而抵制细粒棘球蚴的感染。
目的：觀察TGF-β1對細粒棘毬蚴體外與BALB／c小鼠T淋巴細胞培養的影響。方法：阻斷劑組：BALB／c小鼠脾細胞＋細粒棘毬蚴＋SB525334共培養；對照組：BALB／c小鼠脾細胞＋細粒棘毬蚴＋PBS共培養；空白對照：BALB／c小鼠脾細胞＋RPMI-1640培養基＋SB525334共培養。方法：48 h收集淋巴細胞，流式細胞儀檢測T淋巴細胞亞群、CD4＋CD25＋T細胞的數量、NK細胞的數量及其活性受體NKG2D的錶達；乳痠脫氫酶法檢測NK細胞的殺傷活性。結果：體外阻斷TGF-β1受體後，引起細粒棘毬蚴介導的CD4＋T細胞數量升高、CD8＋T細胞數量降低、CD4＋／CD8＋T細胞比值升高、CD4＋CD25＋T細胞數量減少、NK細胞活性受體NKG2D的錶達增加、NK細胞對靶細胞Yac-1的裂解率增加和NK細胞活性及其活性受體NKG2D成正相關。結論：體外阻斷TGF-β1受體可提高T淋巴細胞免疫，從而牴製細粒棘毬蚴的感染。
목적：관찰TGF-β1대세립극구유체외여BALB／c소서T림파세포배양적영향。방법：조단제조：BALB／c소서비세포＋세립극구유＋SB525334공배양；대조조：BALB／c소서비세포＋세립극구유＋PBS공배양；공백대조：BALB／c소서비세포＋RPMI-1640배양기＋SB525334공배양。방법：48 h수집림파세포，류식세포의검측T림파세포아군、CD4＋CD25＋T세포적수량、NK세포적수량급기활성수체NKG2D적표체；유산탈경매법검측NK세포적살상활성。결과：체외조단TGF-β1수체후，인기세립극구유개도적CD4＋T세포수량승고、CD8＋T세포수량강저、CD4＋／CD8＋T세포비치승고、CD4＋CD25＋T세포수량감소、NK세포활성수체NKG2D적표체증가、NK세포대파세포Yac-1적렬해솔증가화NK세포활성급기활성수체NKG2D성정상관。결론：체외조단TGF-β1수체가제고T림파세포면역，종이저제세립극구유적감염。
Objective:To investigate the effects of TGF-β1 on T lymphocytes of BALB/c mice infected with Echinococcus granulosus( E.granulosus ) in vitro.Methods: The inhibitor group:the spleen cells of BALB/c mouse were co-cultured with E.granulosus and SB525334.The control group:the spleen cells of BALB/c mouse were co-cultured with E.granulosus and PBS.The blank group:the spleen cells of BALB/c mouse were co-cultured with RPMI-1640 medium and SB525334.The lymphocytes were collected at 48 h post-infection.The T lymphocyte subsets, the number of CD4+CD25+T cells, the number of NK cells, and the expression of NKG2D receptor were detected by flow cytometry.The NK cell activity was determined with the lactate dehydrogenase leakage assay(LDH).Results:The inhibition the TGF-β1 receptors resulted in the increase of in the number of CD4+T cells,the decrease in the number of CD8+T cells,the increase of in the ratio CD4+/CD8+T cells,the decrease of in the number of CD4+CD25+T cells,the increase in the expression of the NKG2D receptors,the increase in the lysis rate of Yac-1 cells by NK cells,and a positive cor-relation between the expression of activity receptor NKG2D and killing activity of NK, which were mediated by E.granulosus.Conclusion: The inhibition of TGF-β1 receptors can enhance the immune response of T lymphocytes against E.granulosus infection in vitro.
