中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
5期
632-637
,共6页
田玉慧%袁文清%马倩%杜志敏%李万里%吕路线
田玉慧%袁文清%馬倩%杜誌敏%李萬裏%呂路線
전옥혜%원문청%마천%두지민%리만리%려로선
大豆甙元%抑郁症%BDNF%NPY%免疫调节
大豆甙元%抑鬱癥%BDNF%NPY%免疫調節
대두대원%억욱증%BDNF%NPY%면역조절
Daidzein%Depression%BDNF%NPY%Immune regulation
目的：探讨大豆甙元（ Daidzein）对慢性应激抑郁模型大鼠海马脑源性神经生长因子（ BDNF）、神经肽Y （NPY）蛋白表达和非特异免疫调节作用的影响。方法：取健康成年SD大鼠40只，雄性，体质量（210±19）g，清洁级，用1％蔗糖水喂养4 d，使动物原来的饮水习惯发生改变。第5天开始24 h禁水，但不禁食，第6天给予1％蔗糖水4 h，测1％蔗糖水的偏嗜度。根据1％蔗糖水偏嗜度和体重随机分成4组，每组10只，糖水偏嗜度和体重各组间没有显著差异（ P＞0.05）。正常对照组（ CG）、模型对照组（ MG）、氟西汀对照组（ FG，10.0 mg／kg）、大豆甙元组（ DG，80.0 mg／kg）。造模同时灌胃给药，每日1次，连续给药32 d。造模方法为慢性轻度不可预见性的应激模型加孤养。观察大鼠的行为改变，免疫蛋白印记（ Western blot）技术和免疫组织化学法检测海马BNDF和NPY表达，观察淋巴细胞增值功能，脾脏指数，外周血白细胞数目及抗体分泌细胞功能。结果：与正常对照组相比慢性应急刺激大鼠体重、蔗糖消耗量及大鼠旷场试验得分显著降低，游泳不动时间延长，海马BNDF和NPY表达水平低下，差异有统计学意义（P＜0.01）。氟西汀治疗组和大豆甙元治疗组与模型对照组相比大鼠体重、蔗糖消耗量、大鼠旷场试验得分以及海马BNDF和NPY表达水平显著增加，差异有统计学意义（ P＜0.05或P＜0.01）。大豆甙元组大鼠外周血白细胞数目、抗体分泌细胞功能及淋巴细胞增殖力显著高于模型组。大豆甙元剂量脾脏指数显著高于模型组（ P＜0.01）。结论：大豆甙元能显著拮抗慢性应激模型大鼠抑郁症状，大豆甙元可增加海马内BDNF和NPY蛋白的含量，并且有增强慢性应激模型大鼠体液免疫应答和淋巴细胞增殖能力的作用，大豆甙元拮抗慢性应激模型大鼠抑郁作用的机制可能与大豆甙元治疗后海马中BDNF和NPY蛋白含量增加及免疫调节作用有关。
目的：探討大豆甙元（ Daidzein）對慢性應激抑鬱模型大鼠海馬腦源性神經生長因子（ BDNF）、神經肽Y （NPY）蛋白錶達和非特異免疫調節作用的影響。方法：取健康成年SD大鼠40隻，雄性，體質量（210±19）g，清潔級，用1％蔗糖水餵養4 d，使動物原來的飲水習慣髮生改變。第5天開始24 h禁水，但不禁食，第6天給予1％蔗糖水4 h，測1％蔗糖水的偏嗜度。根據1％蔗糖水偏嗜度和體重隨機分成4組，每組10隻，糖水偏嗜度和體重各組間沒有顯著差異（ P＞0.05）。正常對照組（ CG）、模型對照組（ MG）、氟西汀對照組（ FG，10.0 mg／kg）、大豆甙元組（ DG，80.0 mg／kg）。造模同時灌胃給藥，每日1次，連續給藥32 d。造模方法為慢性輕度不可預見性的應激模型加孤養。觀察大鼠的行為改變，免疫蛋白印記（ Western blot）技術和免疫組織化學法檢測海馬BNDF和NPY錶達，觀察淋巴細胞增值功能，脾髒指數，外週血白細胞數目及抗體分泌細胞功能。結果：與正常對照組相比慢性應急刺激大鼠體重、蔗糖消耗量及大鼠曠場試驗得分顯著降低，遊泳不動時間延長，海馬BNDF和NPY錶達水平低下，差異有統計學意義（P＜0.01）。氟西汀治療組和大豆甙元治療組與模型對照組相比大鼠體重、蔗糖消耗量、大鼠曠場試驗得分以及海馬BNDF和NPY錶達水平顯著增加，差異有統計學意義（ P＜0.05或P＜0.01）。大豆甙元組大鼠外週血白細胞數目、抗體分泌細胞功能及淋巴細胞增殖力顯著高于模型組。大豆甙元劑量脾髒指數顯著高于模型組（ P＜0.01）。結論：大豆甙元能顯著拮抗慢性應激模型大鼠抑鬱癥狀，大豆甙元可增加海馬內BDNF和NPY蛋白的含量，併且有增彊慢性應激模型大鼠體液免疫應答和淋巴細胞增殖能力的作用，大豆甙元拮抗慢性應激模型大鼠抑鬱作用的機製可能與大豆甙元治療後海馬中BDNF和NPY蛋白含量增加及免疫調節作用有關。
목적：탐토대두대원（ Daidzein）대만성응격억욱모형대서해마뇌원성신경생장인자（ BDNF）、신경태Y （NPY）단백표체화비특이면역조절작용적영향。방법：취건강성년SD대서40지，웅성，체질량（210±19）g，청길급，용1％자당수위양4 d，사동물원래적음수습관발생개변。제5천개시24 h금수，단불금식，제6천급여1％자당수4 h，측1％자당수적편기도。근거1％자당수편기도화체중수궤분성4조，매조10지，당수편기도화체중각조간몰유현저차이（ P＞0.05）。정상대조조（ CG）、모형대조조（ MG）、불서정대조조（ FG，10.0 mg／kg）、대두대원조（ DG，80.0 mg／kg）。조모동시관위급약，매일1차，련속급약32 d。조모방법위만성경도불가예견성적응격모형가고양。관찰대서적행위개변，면역단백인기（ Western blot）기술화면역조직화학법검측해마BNDF화NPY표체，관찰림파세포증치공능，비장지수，외주혈백세포수목급항체분비세포공능。결과：여정상대조조상비만성응급자격대서체중、자당소모량급대서광장시험득분현저강저，유영불동시간연장，해마BNDF화NPY표체수평저하，차이유통계학의의（P＜0.01）。불서정치료조화대두대원치료조여모형대조조상비대서체중、자당소모량、대서광장시험득분이급해마BNDF화NPY표체수평현저증가，차이유통계학의의（ P＜0.05혹P＜0.01）。대두대원조대서외주혈백세포수목、항체분비세포공능급림파세포증식력현저고우모형조。대두대원제량비장지수현저고우모형조（ P＜0.01）。결론：대두대원능현저길항만성응격모형대서억욱증상，대두대원가증가해마내BDNF화NPY단백적함량，병차유증강만성응격모형대서체액면역응답화림파세포증식능력적작용，대두대원길항만성응격모형대서억욱작용적궤제가능여대두대원치료후해마중BDNF화NPY단백함량증가급면역조절작용유관。
Objective:To investigate the effects of Daidzein on behavior of chronic stress depression rats and the expression of hippocampal brain-derived neurotrophic factor ( BDNF ) , neuropeptide Y ( NPY ) and non-specific immune regulation.Methods: 40 healthy adult male SD rats with body weight(210±19)g,clean grade,were chosen and fed with 1%sucrose solution for 4 d to change drinking habits.On the fifth day rats were subjected to water deprivation for 24 h without fasting.On the sixth day rats were fed with 1%surcrose solution.4 h later, preference of 1% surcrose solution was examined.According to the 1% sucrose solution preference and weight rats were randomly divided into 4 groups,normal control group(CG),model control group,(MG),fluoxetine group(FG,10.0 mg/kg),daidzein group(DG,80.0 mg/kg).At the same time of establishing model,rats were administered orally once a day for 32 d.The depression model was established by chronic unpredictable mild stress model and separation.The behavioral changes of the rats were observed, and expression of BNDF in hippocampus and NPY was measured by Western blot technology and immunohistochemistry.It was observed the proliferation function of lymphocytes,spleen index,the number of peripheral blood leukocytes and antibody-secreting cell function.Results: Compared with the normal control group(CG),the weight of rats with chronic stress protocol was lower, 1%sucrose consumption decreased,scores of rats in the open field test dropped significantly,the immobility time in the forced swimming test prolonged,the level of expression of BNDF and NPY decreased,all the differences above were statistically sig-nificant(P<0.01).Compared with model group,weight of rats in fluoxetine treatment group(FG) and daidzein treatment group(DG)in-creased,sugar consumption,scores in the open field test and the levels of expression of BNDF and NPY significantly increased,the differences were statistically significant( P<0.05 or P<0.01 ).The number of peripheral blood leukocytes and antibody-secreting cell function and proliferation of lymphocytes force in daidzein treatment group was significantly higher than the model group,daidzein dose spleen index was significantly higher than the model group(P<0.01).Conclusion: The daidzein can antagonize depressive symptoms in chronic stress mice,daidzein may increased content of BDNF in hippocampus and NPY protein, and enhanced the role of humoral immune response and lymphocyte proliferation in rats with chronic stress model.The mechanisms of antidepressant effects of daidzein might be related to the increase of content of BDNF in hippocampus and NPY protein and non -specific immune regulation.
