CAJ | 학술논문

目的：辅助生殖技术会引起子代缺陷，文中通过比较经体外受精（invitro fertilization， IVF）的新鲜胚胎与玻璃化冷冻胚胎移植后所产生的子代小鼠生长状况，以探讨胚胎冷冻对IVF后代生长发育的影响。方法实验共分为3组，对照组（12只）：小鼠自然交配所产生子代；鲜胚移植组（9只）：收集IVF后的小鼠囊胚，直接将囊胚进行移植；冻胚移植组（9只）：收集IVF后的小鼠囊胚，进行玻璃化冷冻胚胎，复苏后移植到假孕母鼠子宫内，分析比较3组子代小鼠体重的变化，性成熟期体脂。结果鲜胚移植组与冻胚移植组胚胎在高倍显微镜下形态无明显差异。冻胚移植组0～12周子代小鼠体重高于对照组（P＜0．01），鲜胚移植组子代小鼠0周、2～6周体重高于对照组（P＜0．05）；冻胚移植组性成熟期脂肪含量[（9．32±4．34）g]高于鲜胚组[（5．24±2．56）g]和对照组[（2．80±0．48）g]，差异有统计学意义（P＜0．05）。冻胚移植组肌肉含量[（33．77±4．22）g]高于对照组[（27．50±1．79）g]，差异有统计学意义（P＜0．01）。结论鲜胚移植技术对子代的生长状况干扰小于玻璃化冷冻技术。
목적：보조생식기술회인기자대결함，문중통과비교경체외수정（invitro fertilization， IVF）적신선배태여파리화냉동배태이식후소산생적자대소서생장상황，이탐토배태냉동대IVF후대생장발육적영향。방법실험공분위3조，대조조（12지）：소서자연교배소산생자대；선배이식조（9지）：수집IVF후적소서낭배，직접장낭배진행이식；동배이식조（9지）：수집IVF후적소서낭배，진행파리화냉동배태，복소후이식도가잉모서자궁내，분석비교3조자대소서체중적변화，성성숙기체지。결과선배이식조여동배이식조배태재고배현미경하형태무명현차이。동배이식조0～12주자대소서체중고우대조조（P＜0．01），선배이식조자대소서0주、2～6주체중고우대조조（P＜0．05）；동배이식조성성숙기지방함량[（9．32±4．34）g]고우선배조[（5．24±2．56）g]화대조조[（2．80±0．48）g]，차이유통계학의의（P＜0．05）。동배이식조기육함량[（33．77±4．22）g]고우대조조[（27．50±1．79）g]，차이유통계학의의（P＜0．01）。결론선배이식기술대자대적생장상황간우소우파리화냉동기술。
Objective Assisted reproductive technology ( ART) is associated with an increased incidence of offspring de-fects.The aim of this study was to investigate the impact of embryo vitrification in in vitro fertilization ( IVF) on the growth and devel-opment of the offspring by comparing the development status of mouse offspring from fresh embryo versus vitrified embryo transplanta-tion. Methods This study included three groups of mouse offspring , from natural fertilization ( n=12 ) , fresh embryo transplanta-tion (FET, n=9), and vitrified embryo transplantation (VET, n=9).We compared the body weight and body fat content in the sex-ually mature stage among the three groups of mice . Results No obvious differences were found in the morphology of the embryos be-tween the FET and VET groups .The body weight of the mouse offspring was significantly higher in the VET than in the natural control group at 0 postnatal (PN) week (P<0.01) and 12 PN weeks (P<0.01), and so was it in the FET group than in the control at 0 PN week (P<0.01) and 6 PN weeks (P<0.05).The body fat content at sexual maturity was remarkably higher in the VET than in the FET and control groups ([9.32 ±4.34] vs [5.24 ±2.56] and [2.80 ± 0.48] g, P<0.05), and so was the muscle content in the VET than in the control ([33.77 ±4.22] vs [27.50 ±1.79] g, P<0.01). Conclusion In IVF, fresh embryo transplantation has less interference with the growth and development of mouse offspring than vitri-fied embryo transplantation .