CAJ | 학술논문

目的：对大鼠C6胶质瘤细胞应用实时荧光定量PCR（ real-time quantitative PCR，qPCR）法观察白细胞介素-12（Interleukin-12，IL-12）家族亚基基因的表达，为今后IL-12家族在脑胶质瘤方面研究奠定基础。方法：提取大鼠C6胶质瘤细胞RNA，反转录成cDNA，应用qPCR法观察IL-12家族亚基基因在大鼠C6胶质瘤细胞mRNA表达影响并进行比较分析。结果：在大鼠C6胶质瘤细胞中，IL-23a，IL-12a的表达丰度为高，EBI3，IL-27的表达丰度为中，IL-12b的表达丰度为低。结论：IL-12家族与胶质瘤发生、发展密切相关，其中IL-12、IL-23是最有潜力治疗脑胶质瘤的细胞因子，其研究进展将为脑胶质瘤的免疫治疗带来新思路。
목적：대대서C6효질류세포응용실시형광정량PCR（ real-time quantitative PCR，qPCR）법관찰백세포개소-12（Interleukin-12，IL-12）가족아기기인적표체，위금후IL-12가족재뇌효질류방면연구전정기출。방법：제취대서C6효질류세포RNA，반전록성cDNA，응용qPCR법관찰IL-12가족아기기인재대서C6효질류세포mRNA표체영향병진행비교분석。결과：재대서C6효질류세포중，IL-23a，IL-12a적표체봉도위고，EBI3，IL-27적표체봉도위중，IL-12b적표체봉도위저。결론：IL-12가족여효질류발생、발전밀절상관，기중IL-12、IL-23시최유잠력치료뇌효질류적세포인자，기연구진전장위뇌효질류적면역치료대래신사로。
Objective:To observe the expression of IL-12 family subunit genes by real-time quantitative PCR in mice C6 glioma cells,construct the basis of the brain glioma research on IL-12 family in the future.Methods:Mice C6 glioma RNA was abstracted and reversed transcription cDNA.The mice C6 glioma cells mRNA expression influence of IL-12 family subunit genes was compared and analyzed by real-time quantitative PCR.Results: In mice C6 glioma cells, high expression abundances in IL-23a, IL-12a, midlde expression abundances in EBI3, IL-27, low expression abundance in IL-12b.Conclusion: IL-12 families are closely related to the occurrence and development of glioma,IL-12,IL-23 are regarded as the most potential anti-glioma cytokines among them,research de-velopments will bring a new way of brain glioma immune therapy.