中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
5期
595-599
,共5页
肺癌%CD4+T细胞%CD8+T 细胞%骨髓源性抑制细胞
肺癌%CD4+T細胞%CD8+T 細胞%骨髓源性抑製細胞
폐암%CD4+T세포%CD8+T 세포%골수원성억제세포
Lung cancer%CD4+T cell%CD8+T cell%MDSC
目的：探讨肺癌小鼠CD4＋T细胞、CD8＋T细胞和骨髓源性抑制细胞（ MDSC）的比例和数量变化。方法：采用LLC细胞皮下接种制备小鼠肺癌肿瘤模型，流式细胞仪检测肿瘤小鼠骨髓、脾脏、淋巴结内CD4＋T细胞、CD8＋T细胞和MDSC的数量变化。结果：与正常对照小鼠相比，肿瘤小鼠脾脏、淋巴结内CD4＋T细胞、CD8＋T细胞的比例和数量显著降低，骨髓CD4＋T细胞变化不明显，但CD8＋T细胞显著减少。 MDSC 在肿瘤小鼠骨髓、脾脏和淋巴结内的比例和数量则显著增加。结论：肺癌小鼠T细胞数量降低而MDSC细胞数量增加，诱导对肿瘤细胞的免疫耐受。
目的：探討肺癌小鼠CD4＋T細胞、CD8＋T細胞和骨髓源性抑製細胞（ MDSC）的比例和數量變化。方法：採用LLC細胞皮下接種製備小鼠肺癌腫瘤模型，流式細胞儀檢測腫瘤小鼠骨髓、脾髒、淋巴結內CD4＋T細胞、CD8＋T細胞和MDSC的數量變化。結果：與正常對照小鼠相比，腫瘤小鼠脾髒、淋巴結內CD4＋T細胞、CD8＋T細胞的比例和數量顯著降低，骨髓CD4＋T細胞變化不明顯，但CD8＋T細胞顯著減少。 MDSC 在腫瘤小鼠骨髓、脾髒和淋巴結內的比例和數量則顯著增加。結論：肺癌小鼠T細胞數量降低而MDSC細胞數量增加，誘導對腫瘤細胞的免疫耐受。
목적：탐토폐암소서CD4＋T세포、CD8＋T세포화골수원성억제세포（ MDSC）적비례화수량변화。방법：채용LLC세포피하접충제비소서폐암종류모형，류식세포의검측종류소서골수、비장、림파결내CD4＋T세포、CD8＋T세포화MDSC적수량변화。결과：여정상대조소서상비，종류소서비장、림파결내CD4＋T세포、CD8＋T세포적비례화수량현저강저，골수CD4＋T세포변화불명현，단CD8＋T세포현저감소。 MDSC 재종류소서골수、비장화림파결내적비례화수량칙현저증가。결론：폐암소서T세포수량강저이MDSC세포수량증가，유도대종류세포적면역내수。
Objective:To investigate the changes of CD4+T cells,CD8+T cells and myeloid derived suppressor cells( MDSC) in immune organs of lung cancer mice.Methods: Lung cancer mouse models were made by subcutaneously injection of Lewis lung cancer cell line( LLC).The CD4+T cell,CD8+T cell and MDSC were detected by flow cytometry.Results:Compared to that of normal control mice,the ratio and numbers of CD4+and CD8+T cell were significantly decreased in the spleen and lymph nodes of lung cancer mice.The number of CD4+T cells had no significant change while CD8+T cells decreased in the bone marrow of lung cancer mice.However,the ratio and number of MDSC were significantly increased in the bone marrow,spleen and lymph nodes of lung cancer mice compared to that of normal control mice.Conclusion: The development of lung cancer leads to decreased T cells and increased MDSC;which may induce immune tolerance to tumor cells.
