CAJ | 학술논문

为进一步研究中药千金子的活性物质，将千金子粉末用乙醇室温浸泡提取，经浓缩、除脂、乙酸乙酯萃取后得到千金子乙酸乙酯萃取物，再经洗涤、重结晶得到单体化合物。通过质谱和核磁共振技术鉴定单体化合物的结构为6，7－二羟基香豆素。以 VC 为阳性对照，采用清除 DPPH 自由基实验法和对猪油的抗氧化实验，研究6，7－二羟基香豆素的抗氧化活性。结果表明，6，7二羟基香豆素清除 DPPH 自由基的体系终浓度 IC50为0．058μg／mL，远低于 VC 的体系终浓度（IC50为0．542μg／mL）；油脂过氧化值实验显示，6，7－二羟基香豆素的抗氧化活性强于 VC。千金子的活性物质6，7－二羟基香豆素具有较强的抗氧化活性。
위진일보연구중약천금자적활성물질，장천금자분말용을순실온침포제취，경농축、제지、을산을지췌취후득도천금자을산을지췌취물，재경세조、중결정득도단체화합물。통과질보화핵자공진기술감정단체화합물적결구위6，7－이간기향두소。이 VC 위양성대조，채용청제 DPPH 자유기실험법화대저유적항양화실험，연구6，7－이간기향두소적항양화활성。결과표명，6，7이간기향두소청제 DPPH 자유기적체계종농도 IC50위0．058μg／mL，원저우 VC 적체계종농도（IC50위0．542μg／mL）；유지과양화치실험현시，6，7－이간기향두소적항양화활성강우 VC。천금자적활성물질6，7－이간기향두소구유교강적항양화활성。
To investigate the active substances in the seeds of euphorbia lathyris,its ethyl acetate extract was obtained after the seeds were powdered,ethanol immersion extracted,condensed,degreased and ex-tracted with ethyl acetate.The monomer compound was obtained after the ethyl acetate extract was washed and recrystallized.The compound was identified as 6,7 -dihydroxycoumarin by mass -spectrometry and nuclear magnetic resonance,whose antioxidant activity was evaluated by DPPH radical scavenging assay and antioxidant experiment on lard.The result showed that the IC50 values of 6,7 -dihydroxycoumarin was 0.058 μg/mL,much lower than that of Vitamine C,0.542 μg/mL.The result of lipid peroxide val-ue test showed that the antioxidant activity of 6,7 -dihydroxycoumarin was stronger than Vitamine C.