临床荟萃
臨床薈萃
림상회췌
CLINICAL FOCUS
2015年
6期
674-676,677
,共4页
闻海丰%冯忠军%韩文龙%秦瑾%于文静
聞海豐%馮忠軍%韓文龍%秦瑾%于文靜
문해봉%풍충군%한문룡%진근%우문정
脊柱炎,强直性%HLA-B27抗原%聚合酶链反应
脊柱炎,彊直性%HLA-B27抗原%聚閤酶鏈反應
척주염,강직성%HLA-B27항원%취합매련반응
spondylitis,ankylosing%HLA-B27 antigen%polymerase chain reaction
目的：构建并评价实时荧光聚合酶链反应(PCR)熔解曲线法检测人类白细胞抗原-B27(HLA-B27)。方法通过采用本研究所构建的实时荧光 PCR 熔解曲线法和微量淋巴细胞毒法、实时荧光定量 PCR 法检测81例标本，并对3种方法进行评价。结果实时荧光 PCR 熔解曲线法与实时荧光定量 PCR 的敏感度和特异度均达到93.8%、90.9%，明显优于微量淋巴细胞毒法的敏感度56.2%和特异度81.8%。结论实时荧光 PCR 熔解曲线法具有很好的敏感度和特异度，具有较好的临床适应性。
目的：構建併評價實時熒光聚閤酶鏈反應(PCR)鎔解麯線法檢測人類白細胞抗原-B27(HLA-B27)。方法通過採用本研究所構建的實時熒光 PCR 鎔解麯線法和微量淋巴細胞毒法、實時熒光定量 PCR 法檢測81例標本，併對3種方法進行評價。結果實時熒光 PCR 鎔解麯線法與實時熒光定量 PCR 的敏感度和特異度均達到93.8%、90.9%，明顯優于微量淋巴細胞毒法的敏感度56.2%和特異度81.8%。結論實時熒光 PCR 鎔解麯線法具有很好的敏感度和特異度，具有較好的臨床適應性。
목적：구건병평개실시형광취합매련반응(PCR)용해곡선법검측인류백세포항원-B27(HLA-B27)。방법통과채용본연구소구건적실시형광 PCR 용해곡선법화미량림파세포독법、실시형광정량 PCR 법검측81례표본，병대3충방법진행평개。결과실시형광 PCR 용해곡선법여실시형광정량 PCR 적민감도화특이도균체도93.8%、90.9%，명현우우미량림파세포독법적민감도56.2%화특이도81.8%。결론실시형광 PCR 용해곡선법구유흔호적민감도화특이도，구유교호적림상괄응성。
Objective To establish and evaluate the technique of the real-time fluorescence quantitative polymerase chain reaction with DNA melting curve analysis for detecting the HLA-B27.Methods Three kinds of methods,including melting curve analysis,micro-complement dependent cytotoxicity test and real-time fluorescence PCR were established.HLA-B27 was detected in 81 patients by the different menthods.Results The sensitivity and specificity of melting curve and real-time fluorescence PCR reached 93.8% and 90.9%,respectively.Those of micro-complement dependent cytotoxicity test reached 56.2% and 81.8%,respectively.Sensitivity and specificity were significantly higher in melting curve and real-time fluorescence PCR than in micro-complement dependent cytotoxicity test.Conclusion Real-time fluorescence PCR with DNA melting curve analysis show high sensitivity and specificity and may be hepful for clinical diagnosis.
