中华眼外伤职业眼病杂志
中華眼外傷職業眼病雜誌
중화안외상직업안병잡지
CHINESE JOURNAL OF OCULAR TRAUMA AND OCCUPATIONAL EYE DISEASE
2015年
4期
241-244
,共4页
李秋明%周欣欣%董洪涛%陈霞
李鞦明%週訢訢%董洪濤%陳霞
리추명%주흔흔%동홍도%진하
白内障,外伤性%皮质,晶状体%浑浊%细胞调亡,晶状体上皮
白內障,外傷性%皮質,晶狀體%渾濁%細胞調亡,晶狀體上皮
백내장,외상성%피질,정상체%혼탁%세포조망,정상체상피
Apoptosis,epithelial cells,lens%Cataract,traumatic%Cortex,lens%Opacity
目的 观察外伤性白内障皮质浑浊对晶状体上皮细胞(LECs)调亡的影响.方法 将牛眼晶状体取出,分为前囊组、后囊组、赤道组和对照组.每组10个晶状体.用1 ml注射器针头将晶状体前囊垂直划破3个2 mm长切口,并搅动刺破处囊下皮质,制成外伤性白内障模型.然后在杜尔贝科改良伊格尔培养基(Dulbecco's modified Eagle's medium,DMEM)中分别培养12h、1、3、6、9和12 d时观察晶状体变化.取晶状体前囊和赤道囊铺片,进行Giemsa染色和原位末端标记(TUNEL)检测,计算LECs凋亡率.结果 牛眼晶状体在损伤后12 h晶状体皮质出现局限性雾状浑浊;6 d时成为完全性浑浊.Giemsa为染色发现随着培养时间的延长,前囊下LECs数量逐渐减少,细胞变小,细胞间隙增宽,边界不清,核固缩逐渐增多.TUNEL染色发现随着皮质浑浊程度的加重和时间的延长,细胞调亡率逐渐增加.对照组无明显变化.结论 在体外培养条件下,牛外伤性白内障晶状体皮质浑浊可诱导LECs的凋亡,细胞凋亡率与皮质浑浊的程度和持续时间呈正相关.
目的 觀察外傷性白內障皮質渾濁對晶狀體上皮細胞(LECs)調亡的影響.方法 將牛眼晶狀體取齣,分為前囊組、後囊組、赤道組和對照組.每組10箇晶狀體.用1 ml註射器針頭將晶狀體前囊垂直劃破3箇2 mm長切口,併攪動刺破處囊下皮質,製成外傷性白內障模型.然後在杜爾貝科改良伊格爾培養基(Dulbecco's modified Eagle's medium,DMEM)中分彆培養12h、1、3、6、9和12 d時觀察晶狀體變化.取晶狀體前囊和赤道囊鋪片,進行Giemsa染色和原位末耑標記(TUNEL)檢測,計算LECs凋亡率.結果 牛眼晶狀體在損傷後12 h晶狀體皮質齣現跼限性霧狀渾濁;6 d時成為完全性渾濁.Giemsa為染色髮現隨著培養時間的延長,前囊下LECs數量逐漸減少,細胞變小,細胞間隙增寬,邊界不清,覈固縮逐漸增多.TUNEL染色髮現隨著皮質渾濁程度的加重和時間的延長,細胞調亡率逐漸增加.對照組無明顯變化.結論 在體外培養條件下,牛外傷性白內障晶狀體皮質渾濁可誘導LECs的凋亡,細胞凋亡率與皮質渾濁的程度和持續時間呈正相關.
목적 관찰외상성백내장피질혼탁대정상체상피세포(LECs)조망적영향.방법 장우안정상체취출,분위전낭조、후낭조、적도조화대조조.매조10개정상체.용1 ml주사기침두장정상체전낭수직화파3개2 mm장절구,병교동자파처낭하피질,제성외상성백내장모형.연후재두이패과개량이격이배양기(Dulbecco's modified Eagle's medium,DMEM)중분별배양12h、1、3、6、9화12 d시관찰정상체변화.취정상체전낭화적도낭포편,진행Giemsa염색화원위말단표기(TUNEL)검측,계산LECs조망솔.결과 우안정상체재손상후12 h정상체피질출현국한성무상혼탁;6 d시성위완전성혼탁.Giemsa위염색발현수착배양시간적연장,전낭하LECs수량축점감소,세포변소,세포간극증관,변계불청,핵고축축점증다.TUNEL염색발현수착피질혼탁정도적가중화시간적연장,세포조망솔축점증가.대조조무명현변화.결론 재체외배양조건하,우외상성백내장정상체피질혼탁가유도LECs적조망,세포조망솔여피질혼탁적정도화지속시간정정상관.
Objective To observe the influence of the cortex opacity on the apoptosis of lens epithelial cells (LECs) after the traumatic cataract.Methods The lenses were taken out from the bovine eyes and divided into the anterior capsular group,the posterior capsular group,the equatorial capsular group and the control group.Each group included ten lenses.The capsule of lenses were punctured into three wounds which were about 2mm with a 1ml disposable needle and the cortex was agitated.Then the lenses were cultured in the DMEM culture fluid for 12 h,1,3,6,9 and 12 d.The lenes were observed and the anterior and equatorial capsule of the lenses were taken out and prepared for Giemsa staining,TUNEL technique and then calculated the rate of apoptosis.Results The bovine lenses appeared local haze and cortex opacity at 12 h after injury and became complete cortex opacity at 6 d.Giemsa staining showed that,with time extension,the number of the epithelial cells on the anterior capsule decreased,the cells became smaller,dilated intercellular space,margin became not distinct,karyopyknosis gradually increased.TUNEL showed that,with the development of lens opacity,brown apoptosis cells gradually increased,while the control group showed no significant change (P ＜ 0.05).Conclusion The lens cortex opacity can induce the apoptosis of LECs in the bovine lens culture traumatic cataract in vitro.The rate of apoptosis is positively correlation to the degree and duration of cortex opacity.
