CAJ | 학술논문

目的：研究负载阿霉素（doxorubicin，DOX）的透明质酸修饰还原敏感纳米粒子HA-Lys-LA对乳腺癌细胞株MDA-MB231的靶向性及其抗肿瘤效果。方法通过流式细胞术检测人乳腺癌细胞株MDA-MB231细胞表面CD44分子的表达，检测对负载DOX的透明质酸修饰还原敏感纳米粒子HA-Lys-LA（DOX-NPs）的摄取情况；利用CCK-8试剂盒检测DOX-NPs对乳腺癌细胞株MDA-MB231杀伤情况；通过划痕实验研究DOX-NPs对乳腺癌细胞株MDA-MB231细胞迁移的抑制情况；利用Hoechst染色法检测DOX-NPs 对MDA-MB231细胞凋亡的诱导情况。结果 DOX-NPs能够被高表达CD44分子的人乳腺癌细胞株MDA-MB231细胞有效摄取；DOX-NPs对MDA-MB231细胞的增殖、迁移具有显著抑制作用，并明显诱导其凋亡。结论负载DOX的透明质酸修饰还原敏感纳米粒子DOX-NPs对人乳腺癌细胞株MDA-MB231细胞具有靶向杀伤及诱导凋亡作用。
목적：연구부재아매소（doxorubicin，DOX）적투명질산수식환원민감납미입자HA-Lys-LA대유선암세포주MDA-MB231적파향성급기항종류효과。방법통과류식세포술검측인유선암세포주MDA-MB231세포표면CD44분자적표체，검측대부재DOX적투명질산수식환원민감납미입자HA-Lys-LA（DOX-NPs）적섭취정황；이용CCK-8시제합검측DOX-NPs대유선암세포주MDA-MB231살상정황；통과화흔실험연구DOX-NPs대유선암세포주MDA-MB231세포천이적억제정황；이용Hoechst염색법검측DOX-NPs 대MDA-MB231세포조망적유도정황。결과 DOX-NPs능구피고표체CD44분자적인유선암세포주MDA-MB231세포유효섭취；DOX-NPs대MDA-MB231세포적증식、천이구유현저억제작용，병명현유도기조망。결론부재DOX적투명질산수식환원민감납미입자DOX-NPs대인유선암세포주MDA-MB231세포구유파향살상급유도조망작용。
Objective To investigate the effect and significance of doxorubicin loaded reversibly crosslinked hyaluronic acid nanoparticles (DOX-NPs) on targeting and inhibiting breast cancer cell MDA-MB231. Methods The expression of CD44 on human breast cancer cell line MDA-MB231 and the uptake of DOX-NPs by MDA-MB231 were measured by flow cy-tomery assay. The proliferation, apoptosis and migration of MDA-MB231 treated with DOX-NPs were determined by CCK-8 assay, Hoechst staining and wound healing experiment, respectively. Results DOX-NPs could be uptaked by highly ex-pressed CD44 of MDA-MB231 cells. DOX-NPs had obvious inhibitional effect on the cells proliferation and migration of MDA-MB231 cells, it could also induce apoptosis. Conclusion DOX-NPs can actively kill the breast cancer cells MDA-MB231 targetly, suppress the proliferation and induce apoptosis.