CAJ | 학술논문

目的：探讨泽兰乙醇提取物对链脲佐菌素（ streptozocin，STZ）诱导糖尿病小鼠肾脏的保护作用及机制。方法小鼠一次性腹腔注射STZ溶液0．12 mg／g诱导糖尿病模型，60 h后将造模成功的小鼠随机分为3组，模型对照组、泽兰乙醇提取物高剂量组[5．0 g／（kg? d）]和泽兰乙醇提取物低剂量组[1．25／（kg? d）]，每组10只，另取正常对照组小鼠10只。泽兰乙醇提取物高、低剂量组灌胃对应剂量泽兰乙醇提取物，正常对照组及模型对照组小鼠灌胃相同体积无菌蒸馏水，灌胃饲养5周后，采用过碘酸－雪弗氏（periodic acid Schiff reaction，PAS）染色法检测各组小鼠肾脏结构，ELISA检测肾组织晚期糖基化终末产物（advanced glycation end－products，AGEs）与转化生长因子β1（transforming growth factor－β1，TGF－β1），同时应用RT－PCR和Western blot法检测各组实验小鼠肾脏单核细胞趋化蛋白－1（monocyte chemotactic protein 1，MCP－1）的表达。结果 PAS染色显示，与模型对照组比较，肾脏结构改变明显。 ELISA结果显示，模型对照组肾脏组织中AGEs、TGF－β1含量均高于正常对照组（ P＜0．01），显著低于模型对照组（ P＜0．05）。RT－PCR结果显示模型对照组 MCP－1 mRNA 表达高于正常对照组（ P＜0．01），泽兰乙醇提取物高、低剂量组低于模型对照组（P＜0．01），低于泽兰乙醇提取物低剂量组（P＜0．05）。 Western blot 结果显示 MCP－1蛋白表达模型对照组高于正常对照组（P＜0．01），但泽兰乙醇提取物高、低剂量组与模型对照组比较差异均无统计学意义。结论泽兰乙醇提取物对STZ诱导糖尿病小鼠肾脏具有保护作用，其机制可能与下调AGEs－MCP－1－TGF－β1表达有关。
목적：탐토택란을순제취물대련뇨좌균소（ streptozocin，STZ）유도당뇨병소서신장적보호작용급궤제。방법소서일차성복강주사STZ용액0．12 mg／g유도당뇨병모형，60 h후장조모성공적소서수궤분위3조，모형대조조、택란을순제취물고제량조[5．0 g／（kg? d）]화택란을순제취물저제량조[1．25／（kg? d）]，매조10지，령취정상대조조소서10지。택란을순제취물고、저제량조관위대응제량택란을순제취물，정상대조조급모형대조조소서관위상동체적무균증류수，관위사양5주후，채용과전산－설불씨（periodic acid Schiff reaction，PAS）염색법검측각조소서신장결구，ELISA검측신조직만기당기화종말산물（advanced glycation end－products，AGEs）여전화생장인자β1（transforming growth factor－β1，TGF－β1），동시응용RT－PCR화Western blot법검측각조실험소서신장단핵세포추화단백－1（monocyte chemotactic protein 1，MCP－1）적표체。결과 PAS염색현시，여모형대조조비교，신장결구개변명현。 ELISA결과현시，모형대조조신장조직중AGEs、TGF－β1함량균고우정상대조조（ P＜0．01），현저저우모형대조조（ P＜0．05）。RT－PCR결과현시모형대조조 MCP－1 mRNA 표체고우정상대조조（ P＜0．01），택란을순제취물고、저제량조저우모형대조조（P＜0．01），저우택란을순제취물저제량조（P＜0．05）。 Western blot 결과현시 MCP－1단백표체모형대조조고우정상대조조（P＜0．01），단택란을순제취물고、저제량조여모형대조조비교차이균무통계학의의。결론택란을순제취물대STZ유도당뇨병소서신장구유보호작용，기궤제가능여하조AGEs－MCP－1－TGF－β1표체유관。
Objective To study the protective effect and mechanism of Lycopus lucidus Turcz ethanol extract on STZ induced diabetic mice kidney.Methods The diabetic mice model were induced by single intraperitoneal injection of 0.12 mg/g STZ.After 60h, the mice successful modeling were divided into model control group,Lycopus lucidus Turcz ethanol extract high-dose group [5.0g/(kg? d)]and low-dose group [1.25/(kg? d)], 10 mice in each group.Another 10 normal mice were used as normal control group.The high-and low-dose group were intragastric administrated corresponding dose Lycopus lucidus Turcz ethanol extract, normal control group and model control group were given the same volume of sterile distilled water.After 5 weeks, the mice kidney structure was observed by periodic acid-Schiff ( PAS ) , advanced glycosylation end products ( AGEs ) and transforming growth factorβ1 (TGF-β1) in kidney tissue were detected by ELISA.The monocyte chemotactic protein-1 (MCP-1) mRNA expression were detected by RT-PCR and MCP-1 protein expression by Western blot in mice kidney.Results PAS staining results showed, compared with model control group,renal structural changes in high-dose group was significantly increased.ELISA results showed AGEs and TGF-β1 content in kidney tissue of model control group were higher than normal control group (P<0.01), the above indexes of high-dose group were lower than model control group (P<0.05) .RT-PCR results showed MCP-1 mRNA expression of model control group was higher than normal control group (P<0.01), MCP-1 mRNA expression of low-and high-dose group model group were lower than model control group (P<0.01), and MCP-1 mRNA expression of high-dose group was lower than low-dose group (P<0.05).Western blot results showed MCP-1 protein expression of model group was higher than normal control group (P<0.01), but there were no significant differences of MCP-1 protein expression between low-or high-dose group model group and model control group. Conclusion Lycopus lucidus Turcz ethanol extract can protect the STZ-induced diabetic mice kidney, and it might be the reason of inhibiting the expression of AGEs-MCP-1-TGF-β1.