CAJ | 학술논문

目的：建立芪术益肝颗粒的质量标准，为控制芪术益肝颗粒质量提供依据。方法：采用薄层色谱法对芪术益肝颗粒中黄芪药材进行鉴别，用高效液相色谱对活性成分黄芪甲苷的含量进行测定，色谱柱为C18柱（4．6mm ×250mm，5μm）；流动相为乙腈－水（40∶60）；进样量为20μL；流速为1．0mL／min；检测器为蒸发光散射检测器，基本参数：氮气流速为1．5 L／min、漂移管温度为55℃。结果：薄层鉴别斑点清晰无干扰，黄芪甲苷在0．052～10．4μg／L范围内呈现良好的线性关系，阴性无干扰，加样回收率为99.8％，RSD值为2．17％。结论：建立的方法操作简单、专属性和重复性良好，可作为芪术益肝颗粒的质量控制方法。
목적：건립기술익간과립적질량표준，위공제기술익간과립질량제공의거。방법：채용박층색보법대기술익간과립중황기약재진행감별，용고효액상색보대활성성분황기갑감적함량진행측정，색보주위C18주（4．6mm ×250mm，5μm）；류동상위을정－수（40∶60）；진양량위20μL；류속위1．0mL／min；검측기위증발광산사검측기，기본삼수：담기류속위1．5 L／min、표이관온도위55℃。결과：박층감별반점청석무간우，황기갑감재0．052～10．4μg／L범위내정현량호적선성관계，음성무간우，가양회수솔위99.8％，RSD치위2．17％。결론：건립적방법조작간단、전속성화중복성량호，가작위기술익간과립적질량공제방법。
Objective:To establish the quality standard of Qizhu Yigan Granule and to provide the basis for the quality control of Qizhu Yigan granule.Methods: TLC was used to identify Radix Astragali from Qizhu Yigan granule for the content of active ingredients of Chinese herbal medicine.Astragaloside was determined by high performance liquid chro-matography, C18 column (4.6mm ×250mm,5μm); acetonitrile water was as mobile phase (40∶60);sample size was for 20μL;the flow rate was 1 .0 mL/min;the basic parameters of detector and evaporative light scattering detection:ni-trogen flow rate was 1 .5 L/min and the drift tube temperature was 55℃.Results:TLC spots were clear without interfer-ence,and astragaloside showed a good linear relationship in the range of 0.052~10.4μg/L,with no negative interfer-ence.The rate of recovery was 99 .8%and RSD value was 2 .17%.Conclusion:The method has a good specificity and repeatability, simple operation, and can be used as the quality control of Qizhu Yigan granule.