中医药学报
中醫藥學報
중의약학보
ACTA CHINESE MEDICINE AND PHARMACOLOGY
2015年
3期
76-78
,共3页
昶国平%魏雅蕾%林宏英%李广雷%刘博譞%蔡伟%张宏桂
昶國平%魏雅蕾%林宏英%李廣雷%劉博譞%蔡偉%張宏桂
창국평%위아뢰%림굉영%리엄뢰%류박현%채위%장굉계
丹参%葛根%正交试验%最佳提取工艺
丹參%葛根%正交試驗%最佳提取工藝
단삼%갈근%정교시험%최가제취공예
Salvia%Pueraria%Orthogonal experiments%Optimized extraction process
目的：筛选最佳的丹葛保肝复方的提取工艺。方法：通过正交试验，利用高效液相色谱法同时测定不同的提取工艺后丹葛保肝复方提取物中丹酚酸B和葛根素的含量，以丹酚酸B和葛根素含量、干浸膏得率为考察指标，考察溶剂用量、提取次数、提取时间对提取效果的影响，优选最佳提取工艺。结果：优选出的最佳工艺为25倍量蒸馏水，浸泡60min，回流提取2次，每次2．5h。结论：优选的提取工艺对各指标性成分的提取率均较高，可以为制剂生产提供依据。
目的：篩選最佳的丹葛保肝複方的提取工藝。方法：通過正交試驗，利用高效液相色譜法同時測定不同的提取工藝後丹葛保肝複方提取物中丹酚痠B和葛根素的含量，以丹酚痠B和葛根素含量、榦浸膏得率為攷察指標，攷察溶劑用量、提取次數、提取時間對提取效果的影響，優選最佳提取工藝。結果：優選齣的最佳工藝為25倍量蒸餾水，浸泡60min，迴流提取2次，每次2．5h。結論：優選的提取工藝對各指標性成分的提取率均較高，可以為製劑生產提供依據。
목적：사선최가적단갈보간복방적제취공예。방법：통과정교시험，이용고효액상색보법동시측정불동적제취공예후단갈보간복방제취물중단분산B화갈근소적함량，이단분산B화갈근소함량、간침고득솔위고찰지표，고찰용제용량、제취차수、제취시간대제취효과적영향，우선최가제취공예。결과：우선출적최가공예위25배량증류수，침포60min，회류제취2차，매차2．5h。결론：우선적제취공예대각지표성성분적제취솔균교고，가이위제제생산제공의거。
Objective:To sort out the best method for the extraction process of Salvia and Pueraria hepatoprotective com-pound.Methods:After orthogonal experiment, use HPLC to simultaneously determine the contents of salvianolic acid B and Puerarin, with the contents of salvianolic acid B and Puerarin, dry extract yields as indexes, the effects of solvent a-mount, extracting times and extracting time on extraction were investigated for the optimum extraction process.Results:The optimum extraction parameters were as follows:25 times of distilled water, soaking 60 min, reflux extraction 2 times and 2.5 h each time.Conclusion:The optimized extraction process for each index component extraction rate is higher and can provide the basis for the preparation of production.
