中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2015年
5期
47-49
,共3页
替加环素%鲍曼不动杆菌%最低抑菌浓度%基因表达
替加環素%鮑曼不動桿菌%最低抑菌濃度%基因錶達
체가배소%포만불동간균%최저억균농도%기인표체
tigecycline%Acinetobacter baumannii%minimal inhibitory concentration%gene expression
目的：探讨替加环素对多重耐药鲍曼不动杆菌adeB基因表达水平的影响。方法收集2012年9月至2014年9月在海南省农垦三亚医院分离的57株多重耐药鲍曼不动杆菌，采用E－test（ Epsilome test）法测定替加环素对多重耐药鲍曼不动杆菌最低抑菌浓度（ minimal inhibitory concentration，MIC），采用PCR扩增技术检测adeB、adeR、adeS外排泵基因分布情况，采用实时荧光RT－PCR技术检测各基因相对表达水平。结果57株多重耐药鲍曼不动杆菌MIC值为0．1～8μg／mL，计算MIC50＝2μg／mL， MIC90＝4μg／mL；其中敏感49株占86．0％，中介5株占8．8％，耐药3株5．2％。将57株细菌按照MIC的范围分为敏感性降低组38株和敏感组19株，敏感性降低组对替加环素敏感菌株30株占78．9％，敏感组对替加环素敏感菌株19株占100．0％，敏感组对替加环素敏感性显著高于敏感性降低组（ P＜0．05）。所有菌株中，共有41株同时检测出adeB、adeR、adeS基因，占86．0％；敏感性降低组检测出adeB、adeR、adeS基因29株占70．7％，敏感组12株占63．2％，2组比较差异无统计学意义；荧光RT－PCR检测结果显示，敏感性降低组adeB基因相对表达量显著低于敏感组（P＜0．05），两组adeR、adeS基因相对表达水平比较差异无统计学意义。结论 AdeB基因在降低多重耐药鲍曼不动杆菌对替加环素的敏感性方面发挥着重要的作用，但是可能还存在adeB以外基因参与调控对替加环素的耐药。
目的：探討替加環素對多重耐藥鮑曼不動桿菌adeB基因錶達水平的影響。方法收集2012年9月至2014年9月在海南省農墾三亞醫院分離的57株多重耐藥鮑曼不動桿菌，採用E－test（ Epsilome test）法測定替加環素對多重耐藥鮑曼不動桿菌最低抑菌濃度（ minimal inhibitory concentration，MIC），採用PCR擴增技術檢測adeB、adeR、adeS外排泵基因分佈情況，採用實時熒光RT－PCR技術檢測各基因相對錶達水平。結果57株多重耐藥鮑曼不動桿菌MIC值為0．1～8μg／mL，計算MIC50＝2μg／mL， MIC90＝4μg／mL；其中敏感49株佔86．0％，中介5株佔8．8％，耐藥3株5．2％。將57株細菌按照MIC的範圍分為敏感性降低組38株和敏感組19株，敏感性降低組對替加環素敏感菌株30株佔78．9％，敏感組對替加環素敏感菌株19株佔100．0％，敏感組對替加環素敏感性顯著高于敏感性降低組（ P＜0．05）。所有菌株中，共有41株同時檢測齣adeB、adeR、adeS基因，佔86．0％；敏感性降低組檢測齣adeB、adeR、adeS基因29株佔70．7％，敏感組12株佔63．2％，2組比較差異無統計學意義；熒光RT－PCR檢測結果顯示，敏感性降低組adeB基因相對錶達量顯著低于敏感組（P＜0．05），兩組adeR、adeS基因相對錶達水平比較差異無統計學意義。結論 AdeB基因在降低多重耐藥鮑曼不動桿菌對替加環素的敏感性方麵髮揮著重要的作用，但是可能還存在adeB以外基因參與調控對替加環素的耐藥。
목적：탐토체가배소대다중내약포만불동간균adeB기인표체수평적영향。방법수집2012년9월지2014년9월재해남성농은삼아의원분리적57주다중내약포만불동간균，채용E－test（ Epsilome test）법측정체가배소대다중내약포만불동간균최저억균농도（ minimal inhibitory concentration，MIC），채용PCR확증기술검측adeB、adeR、adeS외배빙기인분포정황，채용실시형광RT－PCR기술검측각기인상대표체수평。결과57주다중내약포만불동간균MIC치위0．1～8μg／mL，계산MIC50＝2μg／mL， MIC90＝4μg／mL；기중민감49주점86．0％，중개5주점8．8％，내약3주5．2％。장57주세균안조MIC적범위분위민감성강저조38주화민감조19주，민감성강저조대체가배소민감균주30주점78．9％，민감조대체가배소민감균주19주점100．0％，민감조대체가배소민감성현저고우민감성강저조（ P＜0．05）。소유균주중，공유41주동시검측출adeB、adeR、adeS기인，점86．0％；민감성강저조검측출adeB、adeR、adeS기인29주점70．7％，민감조12주점63．2％，2조비교차이무통계학의의；형광RT－PCR검측결과현시，민감성강저조adeB기인상대표체량현저저우민감조（P＜0．05），량조adeR、adeS기인상대표체수평비교차이무통계학의의。결론 AdeB기인재강저다중내약포만불동간균대체가배소적민감성방면발휘착중요적작용，단시가능환존재adeB이외기인삼여조공대체가배소적내약。
Objective To explore the adeB gene expression level impact of tigecycline for multidrug resistance Acinetobacter baumannii.Methods 57 strains multidrug resistance Acinetobacter baumannii were separated from September.2012 to September.2014.Epsilome test method was used to detect minimum inhibitory concentration of tigecycline against multi drug resistant Acinetobacter baumannii.PCR amplification detected adeB, adeR, adeS efflux pump gene distribution.Real-time fluorescent RT-PCR detection technology were used to detect relative expression level of each gene. Results 57 strains of multidrug-resistant Acinetobacter Bauman MIC value were 0.1-8μg/mL.The calculation of MIC50 =2μg/mL, MIC90 =4μg/mL. 49 strains sensitivity accounted for 86.0%, 5 intermediate strains accounted for 8.8%, 3 resistant strains accounted for 5.2%.57 strains of bacteria were divided into 38 strains lower sensitivity group and 19 strains sensitive group, with reduced susceptibility in lower sensitivity group 30 strains, accounting for 78.9%, sensitive group were all sensitive to tigecycline, accounting for 100%, for tigecycline susceptibility sensitive group was significantly higher than that of lower sensitivity group ( P <0.65 ) .41 strains were detected adeB, adeR, adeS gene in all strains, accounted for 86.0%, 29 strains were detected adeB, adeR, adeS genes in lower sensitivity group, accounted for 70.7%,12 strains were detected in sensitive group accounted for 63.2%, the difference between the two groups had no statistical significance.Fluorescent RT-PCR detection results showed that the relative expression of adeB gene with lower susceptibility group was significantly lower than that of sensitive group (P<0.05), relative expression levels of adeR and adeS gene in two groups had no significant difference.Conclusion AdeB gene plays an important role in tigecycline for multidrug resistance Acinetobacter baumannii, but may also exist outside of the adeB gene is involved in the regulation of resistance to tigecycline.
