中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2015年
5期
40-43
,共4页
钱茜%包德明%岳宗进
錢茜%包德明%嶽宗進
전천%포덕명%악종진
PD-1%MG-63%骨肉瘤%干细胞
PD-1%MG-63%骨肉瘤%榦細胞
PD-1%MG-63%골육류%간세포
PD-1%MG-63%osteosarcoma%stem cells
目的：探讨程序性死亡－1（programmed death－1，PD－1）对骨肉瘤细胞MG－63的影响。方法从人骨肉瘤细胞株 MG－63细胞中分选及鉴定骨肉瘤类肿瘤干细胞，MTT法检测PD－1信号对T细胞增殖的影响；RT－PCR检测PD－1mRNA表达。结果1周内癌细胞明显增殖，显示出较强的增殖能力及侵袭性；肿瘤细胞球的形成依赖于血清营养的支持；血清培养基中的MG－63癌细胞增殖数量较无血清悬浮培养的骨肉瘤细胞球细胞明显增高（ P＜0．05）；RT－PCR的结果显示多能干细胞标记CD133在癌细胞球中的表达显著高于MG－63，癌细胞球及MG－63的PD－1表达均明显增加（P＜0．05）。结论 MG－63细胞系具有骨肉瘤干细胞特性，能够表达相应的细胞标记，PD－1的表达亦显著增加，其使患者的免疫功能下调，与肿瘤的发生及进展密切相关。
目的：探討程序性死亡－1（programmed death－1，PD－1）對骨肉瘤細胞MG－63的影響。方法從人骨肉瘤細胞株 MG－63細胞中分選及鑒定骨肉瘤類腫瘤榦細胞，MTT法檢測PD－1信號對T細胞增殖的影響；RT－PCR檢測PD－1mRNA錶達。結果1週內癌細胞明顯增殖，顯示齣較彊的增殖能力及侵襲性；腫瘤細胞毬的形成依賴于血清營養的支持；血清培養基中的MG－63癌細胞增殖數量較無血清懸浮培養的骨肉瘤細胞毬細胞明顯增高（ P＜0．05）；RT－PCR的結果顯示多能榦細胞標記CD133在癌細胞毬中的錶達顯著高于MG－63，癌細胞毬及MG－63的PD－1錶達均明顯增加（P＜0．05）。結論 MG－63細胞繫具有骨肉瘤榦細胞特性，能夠錶達相應的細胞標記，PD－1的錶達亦顯著增加，其使患者的免疫功能下調，與腫瘤的髮生及進展密切相關。
목적：탐토정서성사망－1（programmed death－1，PD－1）대골육류세포MG－63적영향。방법종인골육류세포주 MG－63세포중분선급감정골육류류종류간세포，MTT법검측PD－1신호대T세포증식적영향；RT－PCR검측PD－1mRNA표체。결과1주내암세포명현증식，현시출교강적증식능력급침습성；종류세포구적형성의뢰우혈청영양적지지；혈청배양기중적MG－63암세포증식수량교무혈청현부배양적골육류세포구세포명현증고（ P＜0．05）；RT－PCR적결과현시다능간세포표기CD133재암세포구중적표체현저고우MG－63，암세포구급MG－63적PD－1표체균명현증가（P＜0．05）。결론 MG－63세포계구유골육류간세포특성，능구표체상응적세포표기，PD－1적표체역현저증가，기사환자적면역공능하조，여종류적발생급진전밀절상관。
Objective To discuss the influences of programmed death-1 (PD-1) factor on osteosarcoma cells MG-63.Methods Osteosarcoma stem cells were sovted and identified through osteosarcoma cell strain MG-63 cells.The influence of PD-1 signal on T cells proliferation were detected by MTT. The expression of PD-1 mRNA was detected by RT-PCR.Results Cancer cells had an obvious proliferation within one week, which showed the strong ability of proliferation and aggressivity.The formation of tumor cells spheres depended on the support of serum nutrition.The number of MG-63 cells proliferation in serum culture medium was significantly higher than the osteosarcoma cells spheres in serum-free suspension culture ( P<0.05 ) .Pluripotent stem cell marks that the expression of CD133 in cancer cell sphere was significantly higher than that of MG-63(P<0.05).RT-PCR results showed the PD-1 expression level of cancer cell sphere and MG-63 were increased significantly.Conclusion MG-63 cell line has the character istics of osteosarcoma stem cells.MG-63 cell line can express the corresponding cell markers.The expression of PD-1 also increase significantly which can reduce immune function of patients and is closely related with the occurrence and development of tumors.
