CAJ | 학술논문

目的：研究乌司他丁对储存期悬浮红细胞凋亡损伤的影响。方法采集16名健康自愿者血液并按血库常规处理得悬浮红细胞，同一来源的4份悬浮红细胞中分别添加等量生理盐水（对照组）及5，000、10，000、50，000 U／mL的乌司他丁溶液（试验组C1、C2、C3），在储存0、7、14、21、28及35 d取样检测。使用流式细胞仪检测红细胞体积、磷脂酰丝氨酸（phosphatidylserine，PS）外翻率及细胞内Ca2＋浓度。结果各组红细胞PS外翻率均自14 d开始升高（ P＜0．05）。对照组及C1、C2组红细胞自21d开始明显缩小，而C3组红细胞体积直到28 d才明显缩小，但差异无统计学意义。对照组和C1组细胞内Ca2＋浓度自35 d开始显著增高（ t＝16．33， t＝14．66，P＜0．05），而试验组细胞内Ca2＋浓度均自14 d开始增高；而自21～35 d，C2及C3组细胞内Ca2＋浓度与对照组相比，差异均无统计学意义。结论在储存期内，悬浮红细胞发生凋亡损伤并随着时间延长而加剧，在储存液里添加适量乌司他丁可在一定程度上抑制凋亡损伤。
목적：연구오사타정대저존기현부홍세포조망손상적영향。방법채집16명건강자원자혈액병안혈고상규처리득현부홍세포，동일래원적4빈현부홍세포중분별첨가등량생리염수（대조조）급5，000、10，000、50，000 U／mL적오사타정용액（시험조C1、C2、C3），재저존0、7、14、21、28급35 d취양검측。사용류식세포의검측홍세포체적、린지선사안산（phosphatidylserine，PS）외번솔급세포내Ca2＋농도。결과각조홍세포PS외번솔균자14 d개시승고（ P＜0．05）。대조조급C1、C2조홍세포자21d개시명현축소，이C3조홍세포체적직도28 d재명현축소，단차이무통계학의의。대조조화C1조세포내Ca2＋농도자35 d개시현저증고（ t＝16．33， t＝14．66，P＜0．05），이시험조세포내Ca2＋농도균자14 d개시증고；이자21～35 d，C2급C3조세포내Ca2＋농도여대조조상비，차이균무통계학의의。결론재저존기내，현부홍세포발생조망손상병수착시간연장이가극，재저존액리첨가괄량오사타정가재일정정도상억제조망손상。
Objective To investigate influence of ulinastation in storage period on apoptosis of suspended erythrocyte.Methods RBCs were treated with saline (control group) and different doses of ulinastatin (5,000, 10,000 and 50,000 U/mL in group C1, C2 and C3, respectively).samples were detected when stored at 0,7,14,21,28,35 d,respectively.Indicators of corpuscular volume,phosphatidylserine extroversion rate and intracellular Ca2 +concentration were analyzed by flow cytometer.Results The phosphatidylserine (PS)-exposure levels of 4 groups started to increase on 14 day(P<0.05). Cells of the control group, group C1 and C2 began to shrink remarkably on day 21, while that of Group C3 on 28 day.The intracellular Ca2 +levels of the control group and group C1 started to increase significantly on day 35, (t=16.33,t=14.66,P<0.05).one Ca2 +levels of group C1,C2 and C3 increased on day 14.From 21 to 35 day, the intracellular Ca2 +levels of group C2 and C3 were no significant compared with control group.Conclusion During the storage period, suspended erythrocyteapoptosis increase with time prolonged, adding suitable amount of ulinastatin in stock solution can inhibit apoptosis in damage at some level.