中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2015年
5期
10-13
,共4页
富硒化%博落回%血根碱%抑菌效果
富硒化%博落迴%血根堿%抑菌效果
부서화%박락회%혈근감%억균효과
selenium-enriched%Macleya cordata%sanguinarine%antibacterial effect
目的：研究富硒化对博落回姚效成分抑菌效果的影响。方法以青霉素钠、生理盐水为对照，采用琼脂扩散纸片法检测各富硒化博落回组处理9种常见细菌的平均抑菌圈直径；以20％甲醇、生理盐水作为对照，用20％的甲醇将平均抑菌圈最小的富硒化博落回组，无硒处理博落回组和青霉素钠组分别稀释，使浓度均为0．48、0．24、0．12、0．06、0．03 mg／mL，采用琼脂平板稀释法测定其MIC，评价对其9种常见细菌的抑制作用。结果富硒化处理能大幅提高博落回生物碱的抑菌效果，100μg／mL亚硒酸钠处理组的平均抑菌圈最小（15．33 mm）；富硒化博落回生物碱对大肠杆菌O1、大肠杆菌P22、都伯林大肠杆菌的最低抑菌浓度为从原来0．96 mg／mL降低至0．24 mg／mL；对鼠伤寒杆菌、猪霍乱杆菌的最低抑菌浓度为0．12 mg／mL；对金黄色葡萄球菌、猪链球菌的最低抑菌浓度为0．06 mg／mL；对枯草芽孢杆菌和李氏杆菌的最低抑菌浓度小于0．03 mg／mL。结论富硒化显著改善博落回的抑菌效果，为今后富硒博落回的开发利用，奠定了理论基础。
目的：研究富硒化對博落迴姚效成分抑菌效果的影響。方法以青黴素鈉、生理鹽水為對照，採用瓊脂擴散紙片法檢測各富硒化博落迴組處理9種常見細菌的平均抑菌圈直徑；以20％甲醇、生理鹽水作為對照，用20％的甲醇將平均抑菌圈最小的富硒化博落迴組，無硒處理博落迴組和青黴素鈉組分彆稀釋，使濃度均為0．48、0．24、0．12、0．06、0．03 mg／mL，採用瓊脂平闆稀釋法測定其MIC，評價對其9種常見細菌的抑製作用。結果富硒化處理能大幅提高博落迴生物堿的抑菌效果，100μg／mL亞硒痠鈉處理組的平均抑菌圈最小（15．33 mm）；富硒化博落迴生物堿對大腸桿菌O1、大腸桿菌P22、都伯林大腸桿菌的最低抑菌濃度為從原來0．96 mg／mL降低至0．24 mg／mL；對鼠傷寒桿菌、豬霍亂桿菌的最低抑菌濃度為0．12 mg／mL；對金黃色葡萄毬菌、豬鏈毬菌的最低抑菌濃度為0．06 mg／mL；對枯草芽孢桿菌和李氏桿菌的最低抑菌濃度小于0．03 mg／mL。結論富硒化顯著改善博落迴的抑菌效果，為今後富硒博落迴的開髮利用，奠定瞭理論基礎。
목적：연구부서화대박락회요효성분억균효과적영향。방법이청매소납、생리염수위대조，채용경지확산지편법검측각부서화박락회조처리9충상견세균적평균억균권직경；이20％갑순、생리염수작위대조，용20％적갑순장평균억균권최소적부서화박락회조，무서처리박락회조화청매소납조분별희석，사농도균위0．48、0．24、0．12、0．06、0．03 mg／mL，채용경지평판희석법측정기MIC，평개대기9충상견세균적억제작용。결과부서화처리능대폭제고박락회생물감적억균효과，100μg／mL아서산납처리조적평균억균권최소（15．33 mm）；부서화박락회생물감대대장간균O1、대장간균P22、도백림대장간균적최저억균농도위종원래0．96 mg／mL강저지0．24 mg／mL；대서상한간균、저곽란간균적최저억균농도위0．12 mg／mL；대금황색포도구균、저련구균적최저억균농도위0．06 mg／mL；대고초아포간균화리씨간균적최저억균농도소우0．03 mg／mL。결론부서화현저개선박락회적억균효과，위금후부서박락회적개발이용，전정료이론기출。
Objective To study on antibacterial effect of active ingredient of se-enriched Macleya cordata.Methods Penicillin sodium and saline as control, detected the average inhibition zone diameter of se-enriched Macleya cordata group ( containing 0.96 mg/mL sanguinarine) on 9 common bacteria with disc agar diffusion method;20% methanol and saline as control, se-enriched Macleya cordata group which the average inhibition zone diameter was minimum, without se-enriched Macleya cordata group and penicillin sodium were diluted with 20% methanol, made concentration of 0.48, 0.24,0.12,0.06, 0.03 mg/mL.minimal inhibitory concentration ( MIC) of each treatment group was determined by agar dilution method, evaluated antibacterial effect of se-enriched Macleaya cordata group.Results se-enriched Macleya cordata group could improve antibacterial effect significantly, the average inhibition zone of 100 μg/mL sodium selenite treatment group was minimum(Φ15.33 mm);MIC of se-enriched Macleya cordata group on E.coli O1 , E.coli P22 , E.Dublin reduced from 0.96 to 0.24 mg/mL;MIC of S.typhimurium and S.suipare were 0.12 mg/mL;MIC of S.aureus and S.suis were 0.06 mg/mL; MIC of E.Dublin and L.murrayi were less than 0.03 mg/mL.Conclusion Se-enriched Macleya cordata improves the antibacterial effect signififcantly, and it lays the theoretical foundation for development and utilization of se-enriched Macleya cordata for future.
