中国癌症杂志
中國癌癥雜誌
중국암증잡지
CHINA ONCOLOGY
2015年
5期
360-364
,共5页
姜玲%曹晓正%李程%肖荞%杨剑锋
薑玲%曹曉正%李程%肖蕎%楊劍鋒
강령%조효정%리정%초교%양검봉
卵巢癌%肿瘤干细胞%FoxO3a%自我更新
卵巢癌%腫瘤榦細胞%FoxO3a%自我更新
란소암%종류간세포%FoxO3a%자아경신
Ovarian cancer%Cancer stem cell%FoxO3a%Self-renewal
背景与目的：肿瘤干细胞的存在是卵巢癌高复发率和高死亡率的原因。本文研究紫花牡荆素(casticin，CAS)对SKOV3细胞系卵巢癌干细胞样细胞(ovarian cancer stem cell like cells，OCSLCs)自我更新能力的影响及其机制。方法：体外培养SKOV3细胞系细胞，干细胞培养基悬浮培养SKOV3得到和扩增卵巢癌球形成细胞，即OCSLCs。蛋白[质]印迹法(Western blot)分析FoxO3a磷酸化水平。FoxO3a特异性siRNA转染抑制FoxO3a蛋白表达，并测定肿瘤球形成率。结果：与亲本细胞相比，OCSLCs高表达磷酸化FoxO3a蛋白，具有更高的肿瘤球形成率[(3.1±0.3)% vs (34.8±6.8)%，P＜0.05]。CAS显著抑制OCSLCs肿瘤球形成能力，并降低FoxO3a磷酸化水平。FoxO3a特异性siRNA转染抑制FoxO3a蛋白表达，拮抗CAS抑制OCSLCs肿瘤球形成作用。结论：降低FoxO3a磷酸化水平参与CAS抑制SKOV3细胞系OCSLCs肿瘤球形成作用。
揹景與目的：腫瘤榦細胞的存在是卵巢癌高複髮率和高死亡率的原因。本文研究紫花牡荊素(casticin，CAS)對SKOV3細胞繫卵巢癌榦細胞樣細胞(ovarian cancer stem cell like cells，OCSLCs)自我更新能力的影響及其機製。方法：體外培養SKOV3細胞繫細胞，榦細胞培養基懸浮培養SKOV3得到和擴增卵巢癌毬形成細胞，即OCSLCs。蛋白[質]印跡法(Western blot)分析FoxO3a燐痠化水平。FoxO3a特異性siRNA轉染抑製FoxO3a蛋白錶達，併測定腫瘤毬形成率。結果：與親本細胞相比，OCSLCs高錶達燐痠化FoxO3a蛋白，具有更高的腫瘤毬形成率[(3.1±0.3)% vs (34.8±6.8)%，P＜0.05]。CAS顯著抑製OCSLCs腫瘤毬形成能力，併降低FoxO3a燐痠化水平。FoxO3a特異性siRNA轉染抑製FoxO3a蛋白錶達，拮抗CAS抑製OCSLCs腫瘤毬形成作用。結論：降低FoxO3a燐痠化水平參與CAS抑製SKOV3細胞繫OCSLCs腫瘤毬形成作用。
배경여목적：종류간세포적존재시란소암고복발솔화고사망솔적원인。본문연구자화모형소(casticin，CAS)대SKOV3세포계란소암간세포양세포(ovarian cancer stem cell like cells，OCSLCs)자아경신능력적영향급기궤제。방법：체외배양SKOV3세포계세포，간세포배양기현부배양SKOV3득도화확증란소암구형성세포，즉OCSLCs。단백[질]인적법(Western blot)분석FoxO3a린산화수평。FoxO3a특이성siRNA전염억제FoxO3a단백표체，병측정종류구형성솔。결과：여친본세포상비，OCSLCs고표체린산화FoxO3a단백，구유경고적종류구형성솔[(3.1±0.3)% vs (34.8±6.8)%，P＜0.05]。CAS현저억제OCSLCs종류구형성능력，병강저FoxO3a린산화수평。FoxO3a특이성siRNA전염억제FoxO3a단백표체，길항CAS억제OCSLCs종류구형성작용。결론：강저FoxO3a린산화수평삼여CAS억제SKOV3세포계OCSLCs종류구형성작용。
Background and purpose:Ovarian cancer is associated with a high recurrence and mortality due to the existence of cancer stem cells. The purpose of this study was to investigate the effect of casticin (CAS) on the capability of self-renewal in ovarian cancer stem cell like cells (OCSLCs) derived from SKOV3 cell line. Methods:SKOV3 cell line cells were cultured in vitro, and OCSLCs were obtained and amplified through suspended culture with conditioned medium of the stem cells. The phosphorylation level of FoxO3a was analyzed using Western blot. The protein expression of FoxO3a was inhibited by FoxO3a speciifc siRNA transfection, and then the ratio of sphere-formation was detected. Results: Compared with parental cells, OCSLCs over-expressed phosphorylated FoxO3a (pFoxO3a) and had elevated ratio of sphere-formation [(3.1±0.3)% vs (34.8±6.8)%, P<0.05]. CAS significantly inhibited the capability of sphere-formation in OCSLCs and down-regulated the expression level of pFoxO3a. And the transfection of FoxO3a speciifc siRNA suppressed the protein expression of FoxO3a and attenuated the inhibitory effect of CAS on the sphere-formation of OCSLCs. Conclusion: Reduced expression level of pFoxO3a is involved in the effect that CAS inhibits sphere-formation of OCSLCs derived from SKOV3 cell line.
