CAJ | 학술논문

目的：探讨磷脂酰肌醇3-激酶(PI3K)信号通路在脂多糖(LPS)诱导RAW264.7细胞表达可溶性髓样细胞触发受体-1(sTREM-1)中的作用。方法培养小鼠巨噬细胞株RAW264.7,采用相同浓度的LPS在不同时间诱导RAW264.7细胞,应用Western blot法分别检测PI3K蛋白表达水平,RT-PCR法检测PI3K mRNA表达水平,酶联免疫吸附(ELISA)法检测细胞培养血清中sTREM-1表达水平。用不同浓度PI3K特异性抑制剂LY294002处理细胞,观察上述指标变化。结果 LPS可时间依赖性地诱导RAW264.7细胞PI3K蛋白、PI3K mRNA的表达；LY294002可浓度依赖性地抑制PI3K蛋白、PI3K mRNA的表达；LY294002阻断PI3K信号转导通路后,LPS对sTREM-1表达的诱导作用受到显著抑制,并且具有剂量依赖性。结论 LPS通过PI3K信号通路诱导RAW264.7细胞表达sTREM-1。
목적：탐토린지선기순3-격매(PI3K)신호통로재지다당(LPS)유도RAW264.7세포표체가용성수양세포촉발수체-1(sTREM-1)중적작용。방법배양소서거서세포주RAW264.7,채용상동농도적LPS재불동시간유도RAW264.7세포,응용Western blot법분별검측PI3K단백표체수평,RT-PCR법검측PI3K mRNA표체수평,매련면역흡부(ELISA)법검측세포배양혈청중sTREM-1표체수평。용불동농도PI3K특이성억제제LY294002처리세포,관찰상술지표변화。결과 LPS가시간의뢰성지유도RAW264.7세포PI3K단백、PI3K mRNA적표체；LY294002가농도의뢰성지억제PI3K단백、PI3K mRNA적표체；LY294002조단PI3K신호전도통로후,LPS대sTREM-1표체적유도작용수도현저억제,병차구유제량의뢰성。결론 LPS통과PI3K신호통로유도RAW264.7세포표체sTREM-1。
Objective To investigate the role of the PI3K signaling pathway in sTREM-1 expression of RAW264.7 cells by LPS. Methods RAW264.7 cells of macrophage cell line were cultured,RAW264.7 cells were induced with the same concentration of LPS by different time,PI3K protein expression level was detected by Western blot,PI3K mRNA expression level was detected by the RT-PCR,the expression level of sTREM-1 was detected by enzyme linked im-munosorbent assay method.The RAW264.7 cells were treated with LY294002 by different concentration,the changes of above indexes were observed. Results LPS could induce the PI3K and PI3K mRNA expression of RAW264.7 cells by dependent time.LY294002 could inhibit the PI3K and PI3K mRNA by dependent concentration.After PI3K signal transduction pathway was blocked by LY294002,the sTREM-1 expression was significantly inhibited by dose depen-dent. Conclusion The PI3K signaling pathway is induced in sTREM-1 expression of RAW264.7 cells by LPS.