中国当代医药
中國噹代醫藥
중국당대의약
PERSON
2015年
14期
7-10
,共4页
杨兆林%陈茵%汪莉%赵少锋%邵艳玲
楊兆林%陳茵%汪莉%趙少鋒%邵豔玲
양조림%진인%왕리%조소봉%소염령
α27基因%siRNA%RNA干扰%单纯疱疹病毒Ⅱ型%Vero细胞
α27基因%siRNA%RNA榦擾%單純皰疹病毒Ⅱ型%Vero細胞
α27기인%siRNA%RNA간우%단순포진병독Ⅱ형%Vero세포
α27 gene%siRNA%RNAi%HSV-II%Vero cell
目的:探讨在RNA干扰技术条件上,干扰α27基因表达对单纯疱疹病毒Ⅱ型(HSV-Ⅱ)宿主细胞的影响。方法体外合成四对α27基因特异性siRNA(R1、R2、R3、R4组)和阳性、阴性对照siRNA,用Lipofectamine2000转染Vero细胞后接种HSV-Ⅱ,感染后用MTT比色法分别于12、24、36、48、60、72 h测定细胞的存活情况。结果转染了特异性siRNA的细胞OD值较空白组高,细胞存活情况较好,其中R2和R4组存活情况更好。结论α27基因特异性siRNA对宿主细胞具有保护作用。按照同样设计原则合成的不同序列siRNA,其抑制效果存在明显差异。
目的:探討在RNA榦擾技術條件上,榦擾α27基因錶達對單純皰疹病毒Ⅱ型(HSV-Ⅱ)宿主細胞的影響。方法體外閤成四對α27基因特異性siRNA(R1、R2、R3、R4組)和暘性、陰性對照siRNA,用Lipofectamine2000轉染Vero細胞後接種HSV-Ⅱ,感染後用MTT比色法分彆于12、24、36、48、60、72 h測定細胞的存活情況。結果轉染瞭特異性siRNA的細胞OD值較空白組高,細胞存活情況較好,其中R2和R4組存活情況更好。結論α27基因特異性siRNA對宿主細胞具有保護作用。按照同樣設計原則閤成的不同序列siRNA,其抑製效果存在明顯差異。
목적:탐토재RNA간우기술조건상,간우α27기인표체대단순포진병독Ⅱ형(HSV-Ⅱ)숙주세포적영향。방법체외합성사대α27기인특이성siRNA(R1、R2、R3、R4조)화양성、음성대조siRNA,용Lipofectamine2000전염Vero세포후접충HSV-Ⅱ,감염후용MTT비색법분별우12、24、36、48、60、72 h측정세포적존활정황。결과전염료특이성siRNA적세포OD치교공백조고,세포존활정황교호,기중R2화R4조존활정황경호。결론α27기인특이성siRNA대숙주세포구유보호작용。안조동양설계원칙합성적불동서렬siRNA,기억제효과존재명현차이。
Objective To determine the effect of α27-specific siRNA on host cells infecting by herpes simplex virus typeII (HSV-II). Methods Fourα27-specific siRNAs (R1,R2,R3,R4 group) and the positive,negative control siRNAs were synthesized in vitro by chemical process.The Vero cells were transfected with siRNAs using Lipofectamine2000 followed by HSV-IIinfecting.The activity of the cells were measured at 12,24,36,48,60,72 h by MTT. Results Groups with α27-specific siRNA had higher OD values by MTT colorimetric assay than blank cells,with better survive situa-tion.R2 and R4 group had better survival than other groups. Conclusion α27-specific siRNA can protect host cells of HSV-II.There are obvious differences of inhibition effect of siRNA which are synthesized in accordance with the same priciples.