CAJ | 학술논문

DNA提取是分子生物学研究的基础步骤，而叶样研磨是提取步骤中最为费时费力的环节。该研究探索出一种快速、批量粉碎玉米叶片以提取 DNA的方法，并以 SSR分子标记试验验证此方法在分子标记辅助育种工作的应用的可行性，为大容量样品分子标记试验提供一种快速、批量、成本低、无毒安全的粉碎叶样的方法，提高试验效率。
DNA제취시분자생물학연구적기출보취，이협양연마시제취보취중최위비시비력적배절。해연구탐색출일충쾌속、비량분쇄옥미협편이제취 DNA적방법，병이 SSR분자표기시험험증차방법재분자표기보조육충공작적응용적가행성，위대용량양품분자표기시험제공일충쾌속、비량、성본저、무독안전적분쇄협양적방법，제고시험효솔。
The crushing of leaves is the most time and effort-consuming part in DNA extraction which is a fundamental step in the study of molecular biology. In this study, a new rapid and batch-oriented crushing method for DNA extraction from maize leaves was developed. In addition, the practicability of the developed method in molecular marker-assisted breeding was verified using SSR molecular maker technology so as to provide a rapid, batch-oriented, low-cost and non-toxic leaf-crushing method for a large number of molecular marker tests, improving test effi-ciency.