CAJ | 학술논문

[目的]了解我国猪伪狂犬病病毒流行株 gE基因的遗传变异特性以及其对仔猪的致病性。[方法]利用 Vero 细胞从疑似伪狂犬病病毒（PRV）引起的流产死胎猪的脑组织进行病毒分离。通过PCR进行初步鉴定，对分离株 gE基因序列进行系统进化树分析以及该病毒对6周龄左右仔猪的致病性试验。[结果]成功分离到1株 PRV 毒株，命名为 PRV N5B 株，该病毒能在 Vero 细胞上增值，在15代 TCID50达到107.125/0.1 ml；PCR检测可扩增出特异性条带；gE基因全长1740 bp，系统进化树分析表明分离株与2012年以来新流行的毒株属于同一个相对独立的分支,核苷酸同源性高达99.7%～100%；而与以往发表的国内外相关序列比较，在2个不同的位置分别有3个连续碱基的插入。动物试验表明，2 ml的该病毒（106/0.1 ml）滴鼻接种能使6周龄左右仔猪100%死亡。[结论]该研究中的 PRV N5B分离株 gE基因的遗传变异特性以及其对仔猪的致病性试验对于目前流行的猪伪狂犬病的防控及新的疫苗的研发提供理论依据。
[목적]료해아국저위광견병병독류행주 gE기인적유전변이특성이급기대자저적치병성。[방법]이용 Vero 세포종의사위광견병병독（PRV）인기적유산사태저적뇌조직진행병독분리。통과PCR진행초보감정，대분리주 gE기인서렬진행계통진화수분석이급해병독대6주령좌우자저적치병성시험。[결과]성공분리도1주 PRV 독주，명명위 PRV N5B 주，해병독능재 Vero 세포상증치，재15대 TCID50체도107.125/0.1 ml；PCR검측가확증출특이성조대；gE기인전장1740 bp，계통진화수분석표명분리주여2012년이래신류행적독주속우동일개상대독립적분지,핵감산동원성고체99.7%～100%；이여이왕발표적국내외상관서렬비교，재2개불동적위치분별유3개련속감기적삽입。동물시험표명，2 ml적해병독（106/0.1 ml）적비접충능사6주령좌우자저100%사망。[결론]해연구중적 PRV N5B분리주 gE기인적유전변이특성이급기대자저적치병성시험대우목전류행적저위광견병적방공급신적역묘적연발제공이론의거。
Objective] This study aimed to investigate the genetic variation of gE gene of an epidemic pseudorabies virus (PRV) strain and its pathogenicity to piglets. [Method] By serial passage in Vero cel s, a PRV strain was isolated from the brain tissues of stil born fetuses delivered by sows with suspected PRV infection and preliminarily identified by PCR. gE gene of the isolated PRV strain was ampli-fied and sequenced for phylogenetic analysis. In addition, the pathogenicity of the isolated PRV strain to 6-week-old piglets was evaluated. [Result] A PRV strain was successful y isolated and named PRV N5B strain, which could proliferate in Vero cel s and TCID50 of the 15th generation virus liquid reached 107.125/0.1 ml. Specific bands could be amplified by PCR. gE gene in the isolated PRV strain was 1 740 bp in length. A phylogenetic tree was constructed based on ful-length gE se-quences, which showed that PRV N5B strain and PRV strains isolated since 2012 were clustered into the same independent category and shared 99.7%-100% ho-mology of nucleotide sequences. Compared with related sequences published previ-ously, there were insertions of three consecutive bases at two loci. Animal experi-ments showed that intranasal inoculation of 6-week-old piglets with 2 ml of PRV N5B strain (106/0.1 ml) led to a mortality rate of 100%. [Conclusion] In this study, genetic variability of gE gene in PRV N5B isolate and its pathogenicity to piglets were analyzed, which provided a theoretical basis for the development of new vac-cines to prevent and control porcine pseudorabies.