中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2015年
15期
124-127
,共4页
张彦飞%李智萌%蔡广知%刘凤臣%贡济宇%高文义
張彥飛%李智萌%蔡廣知%劉鳳臣%貢濟宇%高文義
장언비%리지맹%채엄지%류봉신%공제우%고문의
茺蔚子%盐酸水苏碱%高效液相色谱-蒸发光散射检测器法
茺蔚子%鹽痠水囌堿%高效液相色譜-蒸髮光散射檢測器法
충위자%염산수소감%고효액상색보-증발광산사검측기법
Motherwort Fruit%Hydrochloric acid stachydrine%HPLC-ELSD
目的：建立中药茺蔚子中盐酸水苏碱的高效液相色谱-蒸发光散射检测器测定方法。方法 Asahipak NH2P 504E色谱柱(4.6 mm ×250 mm)，流动相为乙腈-0.2%磷酸溶液(84：16)，体积流量1 mL/min，柱温30℃。结果盐酸水苏碱在0.487~4.383μg范围内线性关系良好，r=0.9999，平均回收率为97.96%，RSD为0.92%。结论该法灵敏度高，专属性强，可作为中药茺蔚子中盐酸水苏碱的定量测定方法。
目的：建立中藥茺蔚子中鹽痠水囌堿的高效液相色譜-蒸髮光散射檢測器測定方法。方法 Asahipak NH2P 504E色譜柱(4.6 mm ×250 mm)，流動相為乙腈-0.2%燐痠溶液(84：16)，體積流量1 mL/min，柱溫30℃。結果鹽痠水囌堿在0.487~4.383μg範圍內線性關繫良好，r=0.9999，平均迴收率為97.96%，RSD為0.92%。結論該法靈敏度高，專屬性彊，可作為中藥茺蔚子中鹽痠水囌堿的定量測定方法。
목적：건립중약충위자중염산수소감적고효액상색보-증발광산사검측기측정방법。방법 Asahipak NH2P 504E색보주(4.6 mm ×250 mm)，류동상위을정-0.2%린산용액(84：16)，체적류량1 mL/min，주온30℃。결과염산수소감재0.487~4.383μg범위내선성관계량호，r=0.9999，평균회수솔위97.96%，RSD위0.92%。결론해법령민도고，전속성강，가작위중약충위자중염산수소감적정량측정방법。
Objective To establish HPLC-ELSD determination method of the hydrochloric acid stachydrine in Mother-wort Fruit. Methods The separation by HPLC-ELSD was performed on Asahipak NH2P 50 4E column (4.6 mmí250 mm) with acetonitrile-0.2% phosphoric acid solution (84:16) as mobile phase, 1 mL/min of flow rate and 30℃ of column temperature. Results Hydrochloric acid stachydrine at 0.487-4.383μg had good linear relationship, r=0.9999, the av-erage recovery was 97.96%, RSD was 0.92%. Conclusion The method is sensitive, specific, it can be used as quantita-tive determination method of the hydrochloric acid stachydrine in Motherwort Fruit.
