CAJ | 학술논문

目的：通过对大鼠分别给予 N-正丁基-N-(4-羟基-丁基)亚硝胺[N-butyl-N-(4-hydroxybutyl)nitrosamine,BBN]饮水饲养和 N-甲基亚硝基脲(N-methyl-nitrosourea,MNU)膀胱灌注,来比较两种方法的造模效果以及各自的优缺点。判断 B 型高频超声对大鼠原位膀胱癌的诊断价值。方法 BBN 组大鼠给予0.05％的 BBN 溶液连续喂养6周,后更换为2％的枸橼酸钠溶液连续喂养22周,第28周末为诱导终点。MNU 组大鼠给予 MNU 溶液(20 mg/ml)膀胱灌注,每两周1次,每次0.1 ml/只,连续灌注8周,后再常规喂养6周,第14周为诱癌终点。分别于第28周末(BBN 组)和第14周末(MNU 组)对两组大鼠进行超声扫描诊断,并在处死后取膀胱组织行病理学观察。比较两组的存活率和存活成瘤率。结果 BBN 诱癌组20只大鼠28周后存活11只,成瘤8只,存活率为55％,存活成瘤率为72.7％。MNU 组20只大鼠14周后存活18只,成瘤17只,存活率为90％,存活成瘤率为94.4％。结论利用 MNU 膀胱灌注行大鼠膀胱癌造模,其存活率和存活成瘤率均显著高于BBN 饮水饲养造模。超声可作为大鼠膀胱癌造模活体检测的可靠方法。
목적：통과대대서분별급여 N-정정기-N-(4-간기-정기)아초알[N-butyl-N-(4-hydroxybutyl)nitrosamine,BBN]음수사양화 N-갑기아초기뇨(N-methyl-nitrosourea,MNU)방광관주,래비교량충방법적조모효과이급각자적우결점。판단 B 형고빈초성대대서원위방광암적진단개치。방법 BBN 조대서급여0.05％적 BBN 용액련속위양6주,후경환위2％적구연산납용액련속위양22주,제28주말위유도종점。MNU 조대서급여 MNU 용액(20 mg/ml)방광관주,매량주1차,매차0.1 ml/지,련속관주8주,후재상규위양6주,제14주위유암종점。분별우제28주말(BBN 조)화제14주말(MNU 조)대량조대서진행초성소묘진단,병재처사후취방광조직행병이학관찰。비교량조적존활솔화존활성류솔。결과 BBN 유암조20지대서28주후존활11지,성류8지,존활솔위55％,존활성류솔위72.7％。MNU 조20지대서14주후존활18지,성류17지,존활솔위90％,존활성류솔위94.4％。결론이용 MNU 방광관주행대서방광암조모,기존활솔화존활성류솔균현저고우BBN 음수사양조모。초성가작위대서방광암조모활체검측적가고방법。
Objective To compare the effectiveness of construction of two orthotopic rat mod-els of bladder cancer induced by BBN and MNU,and to evaluate the value of ultrasound in the detec-ting lesions of rats with bladder cancer. Methods 20 female SD rats were given drinking water contain 0.05% BBN for 6 weeks in a row,then the replacement of 2% natrium citrate solution feed 22 weeks continuously.All the rats were evaluated by ultrasound and then killed in the 28 weeks.A total of 20 SD rats was inducted with MNU (0.1 ml per rat)by intravesical administration every other week.At 14 weeks all rats were evaluated by ultrasound and killed. Results BBN treat-ment:there were only 1 1 rats alive and 8 of them had bladder cancer 28 weeks later.All the abnor-mal changes were detected by ultrasound and the result were in accordance with the pathological ex-amination.MNU treatment:18 rats were still living in the 14 weeks.According to the images of ul-trasound ,we found that there were 1 7 rats had tumor. Conclusions The orthotopic bladder cancer model of rat can be induced by BBN and MNU.However,the mortality is lower and the tumorigenic rate is higher which were abducted by MNU than that of BBN treatment.For the orthotopic rat model with bladder cancer,the ultrasound scanning is a reliable technique and it can provide useful help for the further research.