CAJ | 학술논문

近年来，结构生物学研究越来越注重生物大分子复合物的解析，因为许多重要生物学过程都离不开复合物的参与．溶液核磁共振是目前重要的结构解析方法之一．X射线小角散射(SAXS)作为一种新的结构生物学实验手段，近年来发展迅速．SAXS 能提供生物大分子复合物的较低分辨率结构信息，而核磁共振能解析复合物中各个亚基的原子分辨率结构．此外，通过核磁共振还能得到亚基之间的界面、取向以及距离信息．因此近年来通过计算机模拟，整合核磁共振和 SAXS 不同分辨率的结构信息，可以用来搭建生物大分子复合物的结构模型．该综述重点介绍这方面的研究进展．
근년래，결구생물학연구월래월주중생물대분자복합물적해석，인위허다중요생물학과정도리불개복합물적삼여．용액핵자공진시목전중요적결구해석방법지일．X사선소각산사(SAXS)작위일충신적결구생물학실험수단，근년래발전신속．SAXS 능제공생물대분자복합물적교저분변솔결구신식，이핵자공진능해석복합물중각개아기적원자분변솔결구．차외，통과핵자공진환능득도아기지간적계면、취향이급거리신식．인차근년래통과계산궤모의，정합핵자공진화 SAXS 불동분변솔적결구신식，가이용래탑건생물대분자복합물적결구모형．해종술중점개소저방면적연구진전．
Structural biology has been paying more attention on biomolecular complexes over the past decades, since they are crucial for many biological processes. Among these techniques for structural determination, nuclear magnetic resonance (NMR) has its advantage when dealing with biomolecules with high flexibility in solution. Small-angle X-ray scattering (SAXS) is a very important complementary technique that provides information on global shape of biomolecules. For biomolecular complexes, it can be much easier to determine atomic structures of individual subunits through NMR. In addition, NMR can also provide other structural information, such as the interface and orientations between subunits, and long range distance and angular restraints. Therefore, to construct structural models of biomolecular complexes, it would be very appropriate to combine experimental restraints obtained through NMR and low-resolution shape information from SAXS by utilizing computational tools, which is the main topic of this review.